Voprosy meditsinskoi khimii (ISSN 0042-8809)

The optical biosensor technique for detection of hepatitis B virus surface antigen

   


1. Institute of Biomedical Chemistry, Russian Academy of Medical Sciences
2. Russian State Medical University, Chair of children infection disease, Moscow
PubMed Id: 11693030
Year: 2001 vol: 47  issue:4  pages: 419-425
Abstract: A new method for detection of hepatitis B surface antigen (HBsAg) has been developed.It employes IAsys optical biosensor registration kinetics of HBsAg complexes withmonoclonal antibodies. Detection of intermolecular interactions is accompanied by changesof the light refraction coefficient in the sensitive layer of the biosensor cuvette. Themain advantage of this diagnostic technique consists in rapid registration of theseinteractions in real time, without any introduction of special labels into analysingmolecules. The optical biosensor method was successfully employed for the detection ofHBsAg in human blood serum. A comparative study of HBsAg detection by the opticalbiosensor and by immunoenzyme analysis demonstrated high specificity of HBsAg detection bythis new method.
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Reference: Ivanov Yr.D., Gnedenko O.V., Konev V.A., Kovalev O.B., Nikolaeva L.I., Semenova N.V., Uchaikin V.F., Archakov A.I., The optical biosensor technique for detection of hepatitis B virus surface antigen, Voprosy meditsinskoi khimii, 2001, vol: 47(4), 419-425.
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