Voprosy meditsinskoi khimii (ISSN 0042-8809)

Cytotoxic effect of conjugates of L-lysine a-oxidase with monoclonal antibodies on human tumor cell lines

   


1. Blokhin Cancer Research Center of Russian Academy of Medical Sciences
2. Department of Biochemistry, Russian Peoples' Friendship University
PubMed Id: 11925748
Year: 2001 vol: 47  issue:6  pages: 588-592
Abstract: The conjugates of L-lysine a-oxidase and monoclonal antibodies ICO-80 towards CD-5receptor were produced using glutaraldehyde. The cytotoxic effect of conjugates on Yurkatcells line appeared to be lower in comparison with the native enzyme. Negligible decreaseof conjugate biological activity may be explained by the large molecular weight ofconjugate, which is several times higher than the molecular weight of the native enzyme.Such conjugates can not penetrate into the cells. So they catalyze the hydrogen peroxideformation, the main damagihg agent, probably only outside the cells. We suppose also thatthe free native enzyme penetrates into the cell and activates there the oxidativedeamination of L-lysine and correspondingly the hydrogen peroxide formation. This may bethe proper explanation for the higher cytotoxic effect of L-lysinea-?oxidase on Yurkatcell line.
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Reference: Zhukova O.S., Gogichaeva N.V., Lukasheva E.V., Berezov T.T., Cytotoxic effect of conjugates of L-lysine a-oxidase with monoclonal antibodies on human tumor cell lines, Voprosy meditsinskoi khimii, 2001, vol: 47(6), 588-592.
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