Voprosy meditsinskoi khimii (ISSN 0042-8809)

Cloning of the human cytochrome P450 2B6 gene in escherichia coli cells

   


1. Orekhovich Institute of Biomedical Chemistry RAMS
2. Centre 'Bioengineering' RAN
3. Lomonosov Moscow Academy of Fine Chemical Technology
PubMed Id: 11925752
Year: 2001 vol: 47  issue:6  pages: 609-619
Abstract: Human cytochrome Р450 2В6 gene from plasmid pUC9, carrying cytochrome CYP2B6 cDNA was cloned into eucariotic expression vector pcDNA 3.1 (+). Cytochrome P450 2B6 gene in recombinant plasmid pcDNA 3.1 (+) /СУР 2В6 was expressed in E.coli cells. The expression of catalytically active recombinant protein was 60-100 nm per liter of the culture medium
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Reference: Kolomeichuk S.N., Pokrovskaya M.V., Borisova A.A., Jgoun A.A., Solodar L.I., Sokolov N.N., Shvets V.I., Gervaziev Yu.V., Eldarov M.A., Archakov A.I., Cloning of the human cytochrome P450 2B6 gene in escherichia coli cells , Voprosy meditsinskoi khimii, 2001, vol: 47(6), 609-619.
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