Voprosy meditsinskoi khimii (ISSN 0042-8809)

Attachment of biologically active fragments of interferon-a2 and insulin to theartificial protein, albebetin, affects immunogenicity of the resulting construct

   


1. Russian Academy of Sciences Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
2. Russian Academy of Medical Sciences V.N. Orekhovich Institute of Biomedical Chemistry
PubMed Id: 12068502
Year: 2002 vol: 48  issue:1  pages: 94-102
Abstract: The immunogenicity of the artificial protein albebetin and itsderivatives with active peptide fragments was investigated. We also studied the influenceof the peptides on the immunogenicity of the whole construct and contribution of eachcomponent to the immunogenicity. Two of three studied proteins contained active peptidesfrom human IFN-a and insulin. Three continuous antigenic sites with different immunogenicpotential were recognized in the chimerical proteins. The interferon fragment was theimmunodominant site in the albeferon and albeferon-insulin molecules, while the insulinfragment displayed low immunogenic activity. All continuous B-epitopes are located at theboundaries of the secondary structure elements and at the predicted surface-located sitesof albebetin molecule. Thus, peptide fragments attached to the artificial protein carriercan influence immunogenicity of the resulting construct.
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Reference: Bocharova O.V., Moshkovskii S.A., Chertkova R.V., Abdullaev Z.Kh., Kolesanova E.F., Dolgikh D.A., Kirpichnikov M.P., Attachment of biologically active fragments of interferon-a2 and insulin to theartificial protein, albebetin, affects immunogenicity of the resulting construct, Voprosy meditsinskoi khimii, 2002, vol: 48(1), 94-102.
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