Voprosy meditsinskoi khimii (ISSN 0042-8809)

Nitric oxide production by human peripheralblood platelets in normal condition and after thoracic and abdominal wounds

   


1. Sklifosovsky Research Institute for Emergency Medicine (Director - A.S.Ermolov, Prof., RAMS Corr.-Member)
PubMed Id: 12243087
Year: 2002 vol: 48  issue:3  pages: 286-292
Abstract: The study aimed at investigating the nitric oxide production by the human peripheral blood platelets in normal condition and after thoracic and abdominal wounds. The platelets were obtained from blood by differential centrifuging. The nitric oxide production by platelets (0.5х108) was investigated in RPMI 1640 medium containing bovine fetal serum, glutamine, and antibiotics, at the temperature of 37°С for the period of 15 hours. Nitric oxide level was determined by Griss reaction. A large-scale absolute production of nitric oxide by platelets was revealed both in normal condition (7.70±0,55 mmol/L), and after thoracic and abdominal wounds (11.45±0.84 mmol/L). Severe trauma course is associated with hyperproduction of nitric oxide possessing multiple damaging effects, including the anti-thrombogenic one, which should be taken into account in clinical practice
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Reference: Abakumov M.M., Golikov P.P., Nikolaieva N.Yu., Klychnikova E.V., Stotzkaya T.V., Davydov B.V., Matveiev S.B., Nitric oxide production by human peripheralblood platelets in normal condition and after thoracic and abdominal wounds, Voprosy meditsinskoi khimii, 2002, vol: 48(3), 286-292.
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