Voprosy meditsinskoi khimii (ISSN 0042-8809)

Transfection of eucariotic cells using human cytochrome P450 2B6 gene makes them sensitive to cyclophoshamide

   


1. Institute of Biomedical Chemistry RAMS
2. Lomonosov Moscow State Academy of Fine Chemical Technology
3. Centre of Medical Genetics RAMS
PubMed Id: 12498089
Year: 2002 vol: 48  issue:5  pages: 469-476
Abstract: Sensitivity of 293 human epithelialkidney cells transfected by human cytochrome CYP450 gene to cyclophosphamide wasinvestigated. Transfection was carried out by plasmid DNA containing CYP2B6 gene complexedwith cationic liposomes. Liposomes were prepared from mixture of cationic lipids andcholesterol at different molar ratios. Experimental protocol included the following steps:transfection of epithelial kidney cells by complexes of plasmid DNA-cationic liposomes,clone selection in the medium with antibiotic Geneticin G418, selected clone harvestingand their treatment by cyclophosphamide as following cytotoxity evaluation. It was shownthat addition of 0.25 mm of cyclophosphamide resulted in death of 40-45% transfected cellpopulation.
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Reference: Kolomeichuk S.N., Abakumova O.Yu., Maslov M.A., Kalashnikova E.A., Morozova N.G., Serebrennikova G.A., Shvets V.I., Sokolov N.N., Transfection of eucariotic cells using human cytochrome P450 2B6 gene makes them sensitive to cyclophoshamide, Voprosy meditsinskoi khimii, 2002, vol: 48(5), 469-476.
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