Voprosy meditsinskoi khimii (ISSN 0042-8809)

Monoclonal anti-NSE-antibodies: purification,characterization and immunoenzyme analisys

   


1. Serbsky National Research Centre for Social and Forensic Psychiatry
PubMed Id: 12498090
Year: 2002 vol: 48  issue:5  pages: 477-484
Abstract: The results of NSE purificationprocedure, as well as hybridoma technology of anti NSE monoclonal antibodies synthesis arepresented. The employment of this procedure yielded highly purified NSE preparation. Theimmunization of BALB/C mice with NSE preparation led to sensitization of theimmunocompetent cells, which could form hybridomes, producing the anti-NSE monoclonalantibodies, after the confluence with myeloma cells Sp 2/0-Ag 14.
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Reference: Chekhonin V.P., Gurina O.I., Portnaja T.S., Rjabukhin I.A., Shepeleva I.I., Monoclonal anti-NSE-antibodies: purification,characterization and immunoenzyme analisys, Voprosy meditsinskoi khimii, 2002, vol: 48(5), 477-484.
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