Abstract: This is a first report on the presence of creatine kinase, ATP- generating enzyme, in the venom of Vipera xanthia representing the Viperidae family of poisoning snakes. Kinetic and catalytic properties of 40 kD active monomer has been described with a following discussion on the issue of potential of the data listed for further research in either toxic coagulopathy molecular mode or enzyme application dealing with "thrombin - thrombin receptor" interaction studies. An enzyme purification procedure includes hydrophobic (phenyl-Sepharose), anion exchange (MonoQ) and affinity (ADP-Sepharose) chromatographic steps.
Reference: Kouznetsov D.A., Orlova M.A., Berdieva A.G., Khasigov P.Z., ATP-generating creatine kinase: a new component discovered newly in viperidae venom, Biomeditsinskaya khimiya, 2003, vol:
1. Otero R., Nunez V., Jimenez S.L., Fonnegra R., Diaz A. (2000) J. Ethnopharmacol., 71, 505- 511.
2. Nikai Т., Komori Y., Sugihara H. (2000) J. Pharm. Soc. Japan, 120, 315- 327.