ATP-generating creatine kinase: a new component discovered newly in viperidae venom

   


1. Semenov Institute of Chemical Physics, RAS
2. Lomonosov Moscow State University, Russia
3. Sechenov Moscow Medical Academy Russia
Type: Short communication
PubMed Id: 14565086
Year: 2003 vol: 49  issue:2  pages: 213-217
Abstract: This is a first report on the presence of creatine kinase, ATP- generating enzyme, in the venom of Vipera xanthia representing the Viperidae family of poisoning snakes. Kinetic and catalytic properties of 40 kD active monomer has been described with a following discussion on the issue of potential of the data listed for further research in either toxic coagulopathy molecular mode or enzyme application dealing with "thrombin - thrombin receptor" interaction studies. An enzyme purification procedure includes hydrophobic (phenyl-Sepharose), anion exchange (MonoQ) and affinity (ADP-Sepharose) chromatographic steps.
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Reference: Kouznetsov D.A., Orlova M.A., Berdieva A.G., Khasigov P.Z., ATP-generating creatine kinase: a new component discovered newly in viperidae venom, Biomeditsinskaya khimiya, 2003, vol: 49(2), 213-217.
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