Conjugates of pancreatic ribonuclease and ligand -free human serum albumin


1. Pacific Institute of Bioorganic Chemistry , Russian Academy of Sciences
2. Institute of Macromolecular Compounds , Rassion Academy of Sciences, St.Petersburg
3. St. Petersburg Polytechnic University, St. Petersburg
4. Military- medical Academy, St. Petersburg
5. Institute of theoretical and Experimental Biophysics, Rassion Academy of Sciences, Puschino
Type: Experimental/clinical study
PubMed Id: 16119087
Year: 2003 vol: 49  issue:6  pages: 588-596
Abstract: Conjugates of pancreatic RNAse and ligand-free human serum albumin (LFHSA) have been obtained. The number of hydrophobic binding sites both for initial HSA and LFHSA has been determined by the polarised luminescence method. potentiometric titration data has interaction between RNAse and HSA involves additional electrovalent linkage. Unlike initial enzyme, conjugates exhibit activity toward double-strand RNA. After intravenous injection, transferase activity of unmodified enzyme remains in the blood during 20 min., whereas 30-40% of this activity is detected at the fourth day after administration of RNASE conjugates. It have been shown high antiviral activity of LFHSA-RNAse conjugates in once injection in mouse, infected influenza А and influenza В virus.
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Reference: Zelepuga E.A., Ponomareva R.B., Kolikov V.M., Pautov V.D., Sheveleva T.V., Medvedev M.L., Tretyak T.M., Terpelovskaya O.N., Conjugates of pancreatic ribonuclease and ligand -free human serum albumin, Biomeditsinskaya khimiya, 2003, vol: 49(6), 588-596.