Biochemical basis of valproic acid toxicity: role of oxidative stress and effects of L-carnitine


1. Institute of Biochemistry of National Academy of Science of Belarus, Medical University; BLK
2. Medical University; BLK
Type: Experimental/clinical study
PubMed Id: 15518185
Year: 2004 vol: 50  issue:4  pages: 384-389
Abstract: Reduced hepatic mitochondrial β-oxidation and changes in L-carnitine metabolism are important biochemical manifestations of valproate (VA)-induced hepatic toxicity. Lipid peroxidation activation as a possible mechanism implicated in VA - induced damage as well as the possibility of L-carnitine (LC) attenuation of lipid peroxidation activity were studied. The level of malondialdehyde (MDA), lipid peroxide concentration and antioxidant activity (AOA), catalase activity, free S-S groups content in plasma and liver homogenates from male albino rats supplemented with VA (200 mg/kg, 8 days) and VA plus LC (100 mg/kg, 8 days) were measured. There were insignificant differences in MDA formation and catalase activity in the plasma and liver of control and VA - treated groups, however decreases in the plasma AOA activity and S-S groups level were observed in VA-treated rats. The LC administration significantly decreased liver lipid peroxide concentration and increased plasma AOA activity and S-S groups. Our results suggest that lipid peroxidation may be involved as an additional mechanism for VA - induced liver damage in rats. The potential antioxidant activity of LC may be particularly relevant in understanding the pharmacological and biochemical properties of LC in VA - induced pathologic conditions.
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Reference: Bykov I.L., Maltsev A.N., Gurinovich V.A., Nefyodov L.I., Biochemical basis of valproic acid toxicity: role of oxidative stress and effects of L-carnitine, Biomeditsinskaya khimiya, 2004, vol: 50(4), 384-389.