Abstract: A technique for the fluorometric determination of protein and non-protein thiols in brain tissue with the maleimide reagent ThioGlo-1is has been described. Comparison with the most popular spectrophotometric Ellman method has been performed. The ThioGlo-1-based method is highly sensitive and may be used for thiol determination both in homogenates and supernatants of brain tissue.
Reference: Yakovlev A.A., Gulyaeva N.V., Using ThioGlo-1 for the determination of thiols in brain tissue, Biomeditsinskaya khimiya, 2004, vol:
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