Abstract: Aurora B, Which is important for cell division control, is highly expressed in large number of cancer cell lines. Hesperadin, a prototype of a pharmacological agent, is a small molecule inhibitor of catalytic activity of Aurora B. In present work we investigate effect of Hesperadin on breast – MCF7 and prostate adenocarcioma – PC3, cancer cell lines. After Hesperadin treatment we observe stop of cell proliferation due to appearance of multiple mitotic defects caused by Aurora B. Activity reduction and elimination of checkpoint proteins – such as hBUBR1 and CENP-E – fron kinotochores of mitotic chromosomes/
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Reference: Ladygina N.G., Lacis R.V., Yen T., Effects of small molecule hesperadin on breast and prostate cancer cell lines, Biomeditsinskaya khimiya, 2005, vol: 51(2), 170-176.
References
1. Adams R.R., М. Carmena, and Earnshaw W.C. (2001) Trends Cell Biol., 11(2) , 49-54 Scholar google search
2. Hirota Т., Kunitoku N., Sasayama Т., Marumoto Т., Zhang D. (2003) Cell, 114, 585-598 Scholar google search
3. Adams R.R., Helatley W.S., Gouldsworthy A.M., Kandels-Lewis S.E., Carmena M., et.al. (2000) Curr. Biol, 10, 1075-1078 Scholar google search
4. Carmena M., Earnshaw W.C. (2003) Nat. Rev. Mol. Cell Biol., 4, 842-854 Scholar google search
5. Bischoff J.R., Anderson A.L., Zhu Y., Mossie K., Ng L., et. al. (1998) EMBO, 17, 3052-3065 Scholar google search
6. Hauf S., Cole R.W., LaTerra S., Zimmer C., Schnapp G. (2003) J. Cell Biol., 161, 281-294 Scholar google search
7. Chan O.K., Jablonski S.A., Svdakin V., Hittle J.C., Yen T.J. (1999) J. Cell. Biol., 146, 941-954 Scholar google search
8. Adams R.R., Maiato I.I., Earnshaw W.C., Carmena M. (2001) J Cell Biol., 153, 865-880 Scholar google search
9. Jedrusik M.A.and Schulze E. (2004) Methods Mol. Biol., 254, 35-48 Scholar google search
10. Ditchfield C., Johnson V.L., Tighe A., Elision R., Haworth С. (2003) J. Cell Biol., 161, 267-280 Scholar google search
11. Schaar B.T., Chan G.K.T., Maddox R., Salmon E.D., Yen T. (1997) J Cell Biol., 139, 1373-1382 Scholar google search
