Cell mechanisms underlying induction of apoptosis in human erythroleukaemic K562 cells line treated byquinoline-n-oxide derivatives

   


1. Department of Molecular Biology, Petrozavodsk State University
Type: Experimental/clinical study
UDK: 577.27:591.81      PubMed Id: 16805390
Year: 2006 vol: 52  issue:2  pages: 180-187
Abstract: We have studied the influence of 2-(4'-nitrostyryl)-quinoline-1-oxide (2-NSQO) and 4-(4'-nitrostyryl)-quinoline-1-oxide (4-NSQO) on modulation activity of microsomal NADPH-oxidoreductases, concentration of nicotinamide enzymes and induction of apoptosis in human erythroleukaemic K562 cells. It was shown, that activity of microsomal NADPH-cytochrome c-reductases in cancer cells was inhibited by 10 μM 4-NSQO (15%), and 10 μM 2-NSQO (50%). Treatment of cells with these reagents for two days, was accompanied by caspases-9 and -3 activation, rise of EtBr and DAPI fluorescence related to DNA binding and induction of apoptosis. Apoptosis was preceded by the decrease of concentration of nicotinamide enzymes. Thus 4-NSQO is a promising compound for further experimental trials as an anticancer drug with low toxic action on the tissues of organism.
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Reference: Volkova T.O., Zykina N.S., Malycheva I.E., Nemova N.N., Cell mechanisms underlying induction of apoptosis in human erythroleukaemic K562 cells line treated byquinoline-n-oxide derivatives, Biomeditsinskaya khimiya, 2006, vol: 52(2), 180-187.
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