Abstract: Copper-induced oxidability of proteins was investigated in plasma and serum of blood of healthy donors. Incubation of plasma and serum samples with copper ions was accompanied by accumulation of carbonyl products of oxidized proteins. Quantity of the formed carbonyl products depended on both time of incubation, and dilution of plasma and serum. Under identical conditions of oxidation the accumulation of carbonyl products in serum of blood was higher than in plasma.
Reference: Azizova O.A., Piryazev A.P., Moskvina S.N., Aseychev A.V., The method for detection of protein oxidability in serum and plasma of blood, Biomeditsinskaya khimiya, 2007, vol:
1. Sies H. (1985) In: Oxidative stress. Academic Press, London, pp. 1-8.
2. Cherubini A., Ruggiero C., Polidori M.C., Mecocci P. (2005) Free Radic. Biol. Med., 39, 841-852.
3. Berliner J.A., Navab M., Fogelman A.M., Frank J.S., Demer L.L., Edwards P.A., Watson A.D., Lusis A.J. (1995) Circulation, 91, 2488-2496. CrossRef
4. Witztum J.L., Steinberg D. (1991) J. Clin. Invest., 88, 1785-1792. CrossRef
5. Penn M.S., Chisolm G.M. (1994) Atherosclerosis, 108, S21-S29.
6. Koenig W. (1999) Curr. Cardiol. Rep., 1(2), 112-118. CrossRef
7. Shacter E., Williams J.A., Lim M., Levine R.L. (1994) Free Radic. Biol. Med., 17, 429-437.