Effects of (22s,23s)-3β-hydroxy-22,23-oxido-5α-ergost-8(14)-en-15-one and (22r,23r)-3β-hydroxy-22,23-oxido-5α-ergost-8(14)-en-15-one on lipid biosynthesis and exogeneous cholesterol metabolism in hep G2 cells

   


1. Orekhovich Institute of Biomedical Chemistry RAMS
Type: Short communication
UDK: 547.92.057      PubMed Id: 17639725
Year: 2007 vol: 53  issue:2  pages: 221-227
Abstract: Novel synthetic oxysterols (22S,23S)-3β-hydroxy-22,23-oxido-5α-ergost-8(14)-en-15-one (I) and (22R,23R)-3β-hydroxy-22,23-oxido-5α-ergost-8(14)-en-15-one (II) efficiently inhibited cholesterol biosynthesis in human hepatoma Hep G2 cell line at a short time incubation in a serum free medium (IC50 = 1.9±0.2 and 0.6±0.2 µМ, respectively). Cultivation of Hep G2 cells in the presence of compound 5 µM concentration of both (I) and (II), led to significant depression of cholesterol biosynthesis (52% and 57% from control), and remarkable changes in fatty acids, triglycerides, and cholesteryl esters biosynthesis. Compounds (I) and (II) stimulated transformation of exogeneous cholesterol to polar products secreted into the culture medium (156% and 175% from control), that was shown in experiments in Hep G2 cells prelabeled with [3H]cholesterol.
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Reference: Mehtiev A.R., Morozevich G.E., Ivavov V.S., Misharin A.Yu., Effects of (22s,23s)-3β-hydroxy-22,23-oxido-5α-ergost-8(14)-en-15-one and (22r,23r)-3β-hydroxy-22,23-oxido-5α-ergost-8(14)-en-15-one on lipid biosynthesis and exogeneous cholesterol metabolism in hep G2 cells, Biomeditsinskaya khimiya, 2007, vol: 53(2), 221-227.
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