Abstract: Results of experiments on modification of immunostimulating complexes (ISCOM`s) matrix by the replacement of the phospholipid for the glycolipid (monogalactosyldiacylglycerol) from sea macrophytes, and saponin QuillA to triterpene glycoside of сuсumarioside A2-2 from Cucumaria japonica are shown. The resultant complexes include the morphological structures of two types: ISCOM-like structures with the characteristic morphology and sizes and also the tubular structures with diameter of approximately 40 nm and length of 150-400 nm. We have named these structures as TI-complexes. These TI-complexes exhibit considerably lower toxicity than ISCOM. They may include an amphiphilic protein antigen and provide immunoadjuvant effect during experimental vaccination. Under conditions of experimental immunization of mice by a weak immunogen - (subunit membrane pore protein from Y. pseudotuberculosis), TI-complexes with antigen provided stronger humoral immune response to antigen than the complexes of porin with classical ISCOM, liposomes and Freund`s adjuvant. Thus, it`s shown the prospect of the use of TI-complexes as a new type of adjuvant carriers for antigens.
Reference: Tsybulsky A.V., Sanina N.M., Lee I.A., Popov A.M., Kostetsky E.Ja., Portnyagina O.Y., Shnyrov V.L., Elaboration of new adjuvant lipid-saponin complex and its use at experimental immunization by bacterial antigen, Biomeditsinskaya khimiya, 2007, vol:
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