Abstract: Results of experiments on modification of immunostimulating complexes (ISCOM`s) matrix by the replacement of the phospholipid for the glycolipid (monogalactosyldiacylglycerol) from sea macrophytes, and saponin QuillA to triterpene glycoside of сuсumarioside A2-2 from Cucumaria japonica are shown. The resultant complexes include the morphological structures of two types: ISCOM-like structures with the characteristic morphology and sizes and also the tubular structures with diameter of approximately 40 nm and length of 150-400 nm. We have named these structures as TI-complexes. These TI-complexes exhibit considerably lower toxicity than ISCOM. They may include an amphiphilic protein antigen and provide immunoadjuvant effect during experimental vaccination. Under conditions of experimental immunization of mice by a weak immunogen - (subunit membrane pore protein from Y. pseudotuberculosis), TI-complexes with antigen provided stronger humoral immune response to antigen than the complexes of porin with classical ISCOM, liposomes and Freund`s adjuvant. Thus, it`s shown the prospect of the use of TI-complexes as a new type of adjuvant carriers for antigens.
Reference: Tsybulsky A.V., Sanina N.M., Lee I.A., Popov A.M., Kostetsky E.Ja., Portnyagina O.Y., Shnyrov V.L., Elaboration of new adjuvant lipid-saponin complex and its use at experimental immunization by bacterial antigen, Biomeditsinskaya khimiya, 2007, vol:
1. Bomford R. (1990) In: Control of infectious disease. (Dimmock N.J., Griffiths P.D.,Madeley C.R., eds.) Cambridge University Press, Cambridge, pp. 143-154.
2. Babai I., Barenholz Y., Zakay-Rones Z. et al. (2001) Vaccine, 20, 505-515.
3. Lasic D.D. (1995) In: Handbook of Biological Physics Vol.1, (R. Lipowsky, E. Sackmann, eds.), Elsevier Science B.V., pр. 491-519.
4. Van Slooten M.L., Boerman O., Romoren et al. (2001) Biochim. Biophys. Acta, 1530, 134-145.
5. Morein B., Lovgren K., Hoglund S., Sundquist B. (1987) Immunol. Today, 8, 333-338.
6. Morein B., Lovgren K., Hoglund S. (1989) In: Immunological adjuvants and vaccines. (Gregoriadis G. ed.) Plenum Press, New York, pp. 153-162.