Ubiquitin causes selective increase in the sensitivity of rat brain mitochondrial monoamine oxidases to various proteases 1. Institute of Biomedical Chemistry, Russian Academy of Medical Sciences2. School of Biology, Moscow State UniversityType: Short communication UDK: 557.15.02;577.15.06 PubMed Id: 18078075 Year: 2007 vol: 53 issue :5
pages: 603-608 Abstract: Incubation of rat brain mitochondria with ubiquitin and ATP followed by subsequent mitochondria sedimentation was accompanied by reduction of ubiquitin content in the supernatant. This decrease was more pronounced in the presence of ATP-regenerating system in the incubation medium (creatine phosphate/creatine phosphokinase). This ubiquitin incorporation into brain mitochondria observed only in the presence of ATP in the incubation medium increased sensitivity of monoamine oxidases (MAO) A and B to proteolytic inactivation by trypsin and papain, respectively. (Ubiquitin did not influence sensitivity of MAO B to trypsin and MAO A to papain). The data obtained suggest that ubiquitin incorporation into rat brain mitochondria increases susceptibility of MAOs to certain exogenous proteases, however, it remains unclear whether these changes stem from direct MAO-ubiquitin conjugation or reflect alterations in the membrane environment of these enzymes.Download PDF: Reference: Buneeva O.A., Medvedeva M.V., Medvedev A.E., Ubiquitin causes selective increase in the sensitivity of rat brain mitochondrial monoamine oxidases to various proteases, Biomeditsinskaya khimiya, 2007, vol:
53(5), 603-608.This paper is also available as the English translation: 10.1007/s11828-008-1012-1
References
Open 1. Ciechanover A. (1994) Biol. Chem. Hoppe-Seyler, 375, 565-581. Scholar google search 2. Hershko A. (2005) Cell Death Differ., 12(9), 1191-1197. Scholar google search 3. Welchman R.L., Gordon C., Mayer R.J. (2005) Nat. Rev. Mol. Cell Biol., 6(8), 599-609. Scholar google search 4. Varshavsky A. (2005) Trends Biochem. Sci., 30(6), 283-286. Scholar google search 5. Бунеева О.А., Медведев А.Е. (2006) Биохимия, 71(8), 851-860. Scholar google search 6. Ciechanover A., Orian A., Schwartz A.L. (2000) Bioessays, 22, 442-451. Scholar google search 7. Sherman M.Y., Goldberg A.L. (2001) Neuron, 29, 15-32. Scholar google search 8. Hu B.R., Martone M.E., Jones Y.Z., Liu C.L. (2000) J. Neurosci., 20, 3191-3199. Scholar google search 9. Adamo A.M., Pasquini L.A., Moreno M.B., Oteiza P.I., Soto E.F., Pasquini J.M. (1999) J. Neurosci. Res., 55 , 523-531. Scholar google search 10. Mangani M., Serafini G., Antonelli A., Malatesta M., Gazzanelli G. (1991)J. Biol. Chem., 266, 21018-21024. Scholar google search 11. Abell C.W., Kwan S.W. (2001) Progr. Nucleic Acid Res. Mol. Biol., 65, 129-156. Scholar google search 12. Shih J.C., Chen K., Ridd M.J. (1999) Annu. Rev. Neurosci., 22, 197-217. Scholar google search 13. Zhuang Z., McCauley R. (1989) J. Biol. Chem., 264, 14594-14596. Scholar google search 14. Zhuang Z., Marks B., McCauley R. (1992) J. Biol. Chem., 267, 591-596. Scholar google search 15. Medvedev A.E., Gorkin V.Z. (1994) Int. J. Devel. Neurosci., 12, 151-155. Scholar google search 16. Medvedev A.E., Kirkel A., Kamyshanskaya N., Gorkin V. (1993) Int. J. Biochem., 25, 1791-1799. Scholar google search 17. Медведев A.E., Типтон К.Ф. (1997) Вопр. мед. химии, 43, 471-481. Scholar google search 18. Buneeva O.A., Medvedeva M.V., Medvedev A.E. (1999) Neurobiology, 7, 257-261. Scholar google search 19. Barretto N., Jukneliene D., Ratia K., Chen Z., Mesecar A.D., Baker S.C. (2006) Adv. Exper. Med. Biol., 581, 37-41. Scholar google search 20. Sulea T., Linder H.A., Purisima E.O., Menard R. (2005) J. Virol., 79, 4550-4551. Scholar google search 21. Hu M., Li P., Li W., Yao T., Wu J.W., Gu W., Cohen R.E., Shi Y. (2002) Cell, 111, 1041-1054. Scholar google search 22. Wiederanders B. (2003) Acta Biochimica Polonica, 50, 690-713. Scholar google search 23. Dickinson D.P. (2002) Crit. Rev. Oral. Biol. Med., 13, 238-275. Scholar google search 24. Dahlmann B., Kuehn L., Reinauer H. (1995) Biochem. J., 309 ( Pt 1), 195-202. Scholar google search 25. Adachi K., Lakka V., Zhao Y., Surrey S. (2004) J. Biol. Chem., 279, 41767-41774. Scholar google search 26. Gururaja T., Li W., Noble W.S., Payan D.G., Anderson D.C. (2003) J. Proteome, 2, 394-404. Scholar google search 27. Yoshida A., Hirano K., Motoyashiki T., Morita T., Ueki H. (2002) Arch. Biochem. Biophys., 406, 253-260. Scholar google search 28. Dшskeland A.P., Flatmark T. (2002) Eur. J. Biochem., 269, 1561-1569. Scholar google search 29. Sakata N., Dixon J.L. (1999) Biochim. Biophys. Acta, 1437, 71-79. Scholar google search 30. Medvedev A.E., Kirkel A.Z., Kamyshanskaya N.S., Axenova L.N., Moskvitina T.A., Gorkin V.Z., Andreeva N.I., Golovina S.M., Mashkovsky M.D. (1994) Biochem. Pharmacol., 47, 303-308. Scholar google search 31. Laemmli U.K. (1970) Nature (London), 227, 680-685. Scholar google search
