Methods of quantitative proteomics

   


1. Institute of Biomedical Chemistry, Russian Academy of Medical Sciences
Type: Experimental/clinical study
UDK: 577.151      PubMed Id: 18323148
Year: 2007 vol: 53  issue:6  pages: 613-643
Abstract: In modern science proteomic analysis is inseparable from other fields of systemic biology. Possessing huge resources quantitative proteomics operates colossal information on molecular mechanisms of life. Advances in proteomics help researchers to solve complex problems of cell signaling, posttranslational modification, structure and functional homology of proteins, molecular diagnostics etc. More than 40 various methods have been developed in proteomics for quantitative analysis of proteins. Although each method is unique and has certain advantages and disadvantages all these use various isotope labels (tags). In this review we will consider the most popular and effective methods employing both chemical modifications of proteins and also metabolic and enzymatic methods of isotope labeling.
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Reference: Kopylov A.T., Zgoda V.G., Methods of quantitative proteomics, Biomeditsinskaya khimiya, 2007, vol: 53(6), 613-643.
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