Label-free quantitative analysis of proteins using mass-spectrometry


1. Institute of Biomedical Chemistry, RAMS
Type: Experimental/clinical study
UDK: 577.151      PubMed Id: 19507337
Year: 2009 vol: 55  issue:2  pages: 125-139
Abstract: In the present paper we highlight the dependence of normalized mass spectrometry intensity on protein concentration. We have defined dynamic range of concentrations from 1 nM to 1mcM. It has been shown that the dependence holds linearity along the whole concentration range as for pure proteins as for proteins mixtures. Our observation revealed that the correlation curve possess protein-specific tangent between 1 nM and 1 mcM. The efficacy of the method evaluated by AQUA and 18O-labelling techniques of quantitative proteomics.
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Reference: Kopylov A.T., Zgoda V.G., Archakov A.I., Label-free quantitative analysis of proteins using mass-spectrometry, Biomeditsinskaya khimiya, 2009, vol: 55(2), 125-139.
This paper is also available as the English translation:10.1134/S1990750810010075