Visualization and identification of hepatitis c viral particles by atomic force microscopy combined with ms/ms analysis

   


1. Institute of Biomedical Chemistry RAMS
2. Joint Institute for High Temperatures RAS
Type: Experimental/clinical study
DOI: 10.18097/pbmc20105601026      UDK: 577.152.3      PubMed Id: 21328909
Year: 2010 vol: 56  issue:1  pages: 26-39
Abstract: Possibility of detection and identification of hepatitis C viral particles with mass spectrometry (MS) in combination with atomic force microscopy (AFM) had been investigated. AFM/MS approach is based on two technologies: 1. AFM-biospecific fishing that allows to detect, concentrate from solution and to count protein complexes on a surface of AFM-nanochip; 2. mass spectrometric identification of these complexes. AFM-biospecific fishing of HCVcoreAg from solution was carried onto surface of AFM-nanochips with immobilized anti-HCVcoreAg. It was shown that HCVcoreAg/anti-HCVcoreim complexes were formed onto AFM-nanochips in quantity sufficient for mass spectrometric identification. Thus, AFM/MS approach allows to identify fragments of hepatitis C virus fished onto a surface of AFM-nanochip from serum.
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Reference: Kaysheva A.L., Ivanov Yu.D., Zgoda V.G., Frantsuzov P.A., Pleshakova T.O., Krohin N.V., Ziborov V.S., Archakov A.I., Visualization and identification of hepatitis c viral particles by atomic force microscopy combined with ms/ms analysis, Biomeditsinskaya khimiya, 2010, vol: 56(1), 26-39.
This paper is also available as the English translation:10.1134/S1990750810010038
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