Mass-spectrometric identification of interaction sites for cytochrome P450 2b4/nadph cytochrome P450 reductase

   


1. Institute of Biomedical Chemistry, Russian Academy of Medical Sciences
Type: Experimental/clinical study
DOI: 10.18097/pbmc20105601040      UDK: 577.152.3      PubMed Id: 21328910
Year: 2010 vol: 56  issue:1  pages: 40-54
Abstract: We determined the interaction sites of the cytochrome P450's protein-partners: 2B4 (d-2B4) and NADPH-cytochrome P450 of reductase (d-Fp). While in operation, these proteins are forming the complexes. We used 4-4'-dithio(bisphenyl)azide linker for non-specific covalent coupling of d-2B4 complexes with d-Fp in Emulgen-913 - monomerized system. Covalently-linked peptides in this complex were identified with ESI-MS/MS. Several sites of these proteins' binding with each other were revealed. Based on them, a model of intermolecular protein interactions was created. The model includes 5 cross-linker-stabilized contact sites of d-2B4 with d-Fp involving the following peptides of d-2B4 and d-Fp: 1) d-2B4423-433 и d-Fp 102-109; 2) d-2B4324-336 и d-Fp570-585; 3) d-2B4327-336 и d-Fp452-464; 4) d-2B4 192-197 и d-Fp456-464; 5) d-2B4 134-139 и d-Fp406-425.Herein, in the latter two cases, the peptides of d-Fp are located in their inter-domain slit and stabilize protein-protein complex via nanoprobe cross-linker; therefore, the formation of d-2B4/d-Fp complexes in these sites may involve aminoacid residues d-Fp456-464 and d-Fp406-425 surrounding inter-domain slit.
Download PDF:
Reference: Ivanov A.V., Kopylov A.T., Zgoda V.G., Toropygin I.Yu., Khrjapova E.V., Ivanov Yu.D., Mass-spectrometric identification of interaction sites for cytochrome P450 2b4/nadph cytochrome P450 reductase, Biomeditsinskaya khimiya, 2010, vol: 56(1), 40-54.
This paper is also available as the English translation:10.1134/S1990750809040052
Bibliography
 2016(Vol:62)
 2015(Vol:61)
 2014(Vol:60)
 2013(Vol:59)
 2012(Vol:58)
 2011(Vol:57)
 2010(Vol:56)
 2009(Vol:55)
 2008(Vol:54)
 2007(Vol:53)
 2006(Vol:52)
 2005(Vol:51)
 2004(Vol:50)
 2003(Vol:49)