Expression and catalytic properties of rat liver nadp-isocitrate dehydrogenase under normal conditions, after administration of tumor necrosis factor-α and at thioctic acid action

   


1. Department of Medical Biochemistry and Microbiology, Biology and Soil Science Faculty, Voronezh State University
Type: Experimental/clinical study
DOI: 10.18097/pbmc20105602220      UDK: 577.152+577.17: 616.36.002      PubMed Id: 21341510
Year: 2010 vol: 56  issue:2  pages: 220-229
Abstract: Development of apoptosis is accompanied by a decrease in transcripts of NADP-isocitrate dehydrogenase (NADP-IDH, E.C. 1.1.1.42.), and also alterations of catalytic properties from the rat liver enzyme in comparison with control. Administration of thioctic acid is increased the level of expression towards normal values. Enzyme preparations NADP-IDH were obtained from rat liver at norm conditions, after introduction of tumor necrosis factor and thioctic acid action under apoptosis. Molecular weight of homogenous preparations of NADP-IDH purified from livers of control and experimental rats was 112±5,8 kDa, however Km values and pH-optimum changed in apoptosis. Regulation of NADP-IDH activity under effect of some intermediates of the tricarboxylic acid cycle also differed in these groups.
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Reference: Tsvetikova L.N., Popova T.N., Rakhmanova T.I., Iskusnykh I.Y., Expression and catalytic properties of rat liver nadp-isocitrate dehydrogenase under normal conditions, after administration of tumor necrosis factor-α and at thioctic acid action, Biomeditsinskaya khimiya, 2010, vol: 56(2), 220-229.
This paper is also available as the English translation:10.1134/S1990750810020101
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