Investigation of peroxide modification of low- and very low-density lipoproteins in patients with coronary heart disease by the method of fluorescent spectroscopy

   


1. National Center of Cardiology and Internal Medicine, Kirghiz Ministry of Health
Type: Experimental/clinical study
DOI: 10.18097/pbmc20115701127      UDK: 612.123;535.37      PubMed Id: 21516784
Year: 2011 vol: 57  issue:1  pages: 127-132
Abstract: In the total fraction of low and very low density lipoproteins (LDL+VLDL) isolated from serum by precipitation in the presence of heparin-Mn the copper-induced lipid peroxidation was accompanied by accumulation of LPO products, a decrease ANS fluorescence intensity (FANS) and an increase in probe - cation DSP-6, a fluorescence intensity decrease of intrinsic in the ultraviolet area (Fuv) and an increase in the visible area (Fvis). The degree of lipoprotein modification was estimated by calculating the Fvis / Fuv and FDSP-6 / FANS ratio. Strong positive correlation was found between these ratios and concentration of thiobarbituric acid-reactive substances (TBARS) of LDL+VLDL samples isolated from sera of 49 donors and incubated at 37°С in the presence of 50 M CuSO4 during 0, 3 and 24 hr (Fvis / Fuv (r=0,75; p<0,001) and FDSP-6 / FANS (r=0,73; p<0,001)). Very strong positive correlation was also found between both fluorescent parameters Fvis / Fuv and FDSP-6 / FANS (r=0,95, p<0,001). Changes in the values of Fvis / Fuv, FDSP-6 / FANS, concentration of TBARS in 75 patients with documented coronary heart disease (CHD) and 49 apparently healthy donors were studied. No significant differences of these parameters in LDL+VLDL of patients with CHD and donors were found.
Download PDF:
Reference: Aidyraliev R.K., Igemberdieva O.A., Dadabaev M.Kh., Murataliev T.M., Aitbaev K.A., Aldashev A.A., Investigation of peroxide modification of low- and very low-density lipoproteins in patients with coronary heart disease by the method of fluorescent spectroscopy, Biomeditsinskaya khimiya, 2011, vol: 57(1), 127-132.
Bibliography
 2016(Vol:62)
 2015(Vol:61)
 2014(Vol:60)
 2013(Vol:59)
 2012(Vol:58)
 2011(Vol:57)
 2010(Vol:56)
 2009(Vol:55)
 2008(Vol:54)
 2007(Vol:53)
 2006(Vol:52)
 2005(Vol:51)
 2004(Vol:50)
 2003(Vol:49)