A new approach to studying the autoxidation of adrenaline: possibility of the determination of superoxide dismutase activity and the antioxidant properties of various preparations by polarography

   


1. Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences
Type: Experimental/clinical study
DOI: 10.18097/pbmc20125801077      UDK: 577.1:577.3:577.117:577.121:577.13      PubMed Id: 22642154
Year: 2012 vol: 58  issue:1  pages: 77-87
Abstract: The reaction of adrenaline autoxidation in an alkaline buffer with the formation of superoxide radicals and the product of its oxidation, adrenochrome, which models the quinoid pathway of adrenaline conversion in the body, is accompanied by oxygen consumption. This reaction is applicable for polarographic determination of the activity of superoxide dismutase and the antioxidant properties of biological and chemical compounds, it is based on evaluation of the latent period and the rate of oxygen consumption, which are measured in the presence of the compounds examined. It was assumed that the neuro- and cardiotoxicity of quinone products of adrenaline oxidation is related not only to their "own" properties and reactive oxygen species formed but also the hypoxia of those regions of the cell and tissue where the quinoid oxidation of adrenaline occurs.
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Reference: Sirota T.V., A new approach to studying the autoxidation of adrenaline: possibility of the determination of superoxide dismutase activity and the antioxidant properties of various preparations by polarography, Biomeditsinskaya khimiya, 2012, vol: 58(1), 77-87.
This paper is also available as the English translation:10.1134/S1990750811030139
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