Determination of phenobarbital in human urine and serum usingflow injection chemiluminescence

   


1. Northwest University (Sian, China)
Type: Experimental/clinical study
DOI: 10.18097/pbmc20125801088      UDK: 616-07:519.248      PubMed Id: 22642155
Year: 2012 vol: 58  issue:1  pages: 88-94
Abstract: A sensitive chemiluminescence method, based on the enhancive effect of phenobarbital on the chemiluminescence reaction between luminol and dissolved oxygen in a flow injection system, was proposed for the determination of phenobarbital. The chemiluminescence intensity responded to the concentration of phenobarbital linearly ranging from 0.05 to 10 ng⋅ml-1 with the detection limit of 0.02 ng⋅ml-1 (3σ). At a flow rate of 2.0 ml⋅min-1, a complete determination of phenobarbital, including sampling and washing, could be accomplished in 0.5 min, offering the sampling efficiency of 120 h-1 accordingly. The method was applied successfully in an assay of PB for pharmaceutical preparations, human urine and serum without any pretreatment with recovery from 95.7 to 106.7% and RSDs of less than 3.0%.
Download PDF:
Reference: Li K., Nu L.Z., Khe K.L., Song K.H., Determination of phenobarbital in human urine and serum usingflow injection chemiluminescence, Biomeditsinskaya khimiya, 2012, vol: 58(1), 88-94.
This paper is also available as the English translation:10.1134/S1990750811020077
Bibliography
 2016(Vol:62)
 2015(Vol:61)
 2014(Vol:60)
 2013(Vol:59)
 2012(Vol:58)
 2011(Vol:57)
 2010(Vol:56)
 2009(Vol:55)
 2008(Vol:54)
 2007(Vol:53)
 2006(Vol:52)
 2005(Vol:51)
 2004(Vol:50)
 2003(Vol:49)