Abstract: The low-molecular-weight DNA appears in blood plasma of irradiated rats, and its content correlates directly with the irradiation dose. Cloning has shown, that enrichment of low-molecular-weight DNA with G+C content and features of its nucleotide sequences point to its ability to form rather stable nucleosomes. DNA obtained after irradiation of rats with principally different doses 8 and 100 Gy differed not only quantitatively, but also by content of the dinucleotides CpG and CpT; this suggests their origin from different sites of genome. For the first time it has been shown that exposure to low-frequency noise results in an increase of the contents of blood plasma low-molecular-weight DNA. In stroke patients blood concentrations of this DNA increased 3 days after the beginning of the acute period, and dynamics of its excretion differs in ischemic and hemorrhagic forms; in the case of ischemia low-molecular-weight DNA appears in cerebrospinal fluid. The chronic obstructive pulmonary disease in the state of remission is characterized by the decline of the level of low-molecular-weight DNA in the blood plasma unlike in the case of the chronic nonobstructive bronchitis. The clear dependence between formation and special features of the low-molecular-weight DNA fraction in blood plasma makes it possible to consider the low-molecular fraction as an universal index of apoptosis, which allows to distinguish basically different conditions of the body.
Reference: Vasilyeva I.N., Zinkin V.N., The value of low-molecular-weight DNA of blood plasma in the diagnostic of the patological processes of different genesis, Biomeditsinskaya khimiya, 2013, vol:
5. Chen D., Pan Sh., Zhang Sh., Huang P., Xia W., Xie E., Gu B., Wang F., Xu J., Xu T., Lu., Yang D., Lu Sh. (2011) in: Circulating Nucleic Acids in Plasma and Serum (Gahan P.B., ed.) Springer, Dordrecht Heidelberg London New York, pp.171-182. Scholar google search
6. Herrmann M., Leitmann W., Krapf E.F., Kalden J.R. (1989) In: Molecular and Cellular Mechanisms of Human Hypersensitivity and Autoimmunity, N.Y., Vol. 105, pp.147-157. Scholar google search
25. Van der Vaart M., Semenov D.V., Kuligina E.V., Richter V.A., Pretorius P.J. (2011) in: Circulating Nucleic Acids in Plasma and Serum (Gahan P.B., ed.) Springer, Dordrecht Heidelberg London New York, pp. 29-33. Scholar google search
26. Adams R., Burdon R. (1985) Molecular biology of DNA methylation. Springer-Verlag. N.Y. Scholar google search
33. Zhou Y., Dinq D., Zheng H., Zheng G., Shen X., Zhang Q., Salvy R. (2011) Zhonqhua Er Bi Yan Hou Ton Jinq Wai Ke Za Zhi. [Chinese Journal of Otorhinolaryngology Head and Neck Surgery], 46, 54-58. Scholar google search