Irreversible chemical afm-fishing for the detection of low-copied proteins


1. Orekhovich institute of biomedical chemistry of the Russian academy of medical sciences
2. NRC “Kurchatov institute”
3. Joint Institute for High Temperatures of Russian Academy of Sciences
DOI: 10.18097/pbmc20146001028      UDK: 577.1      PubMed Id: 24749246
Year: 2014 vol: 60  issue:1  pages: 28-50
Abstract: The atomic-force microscopy-based method of irreversible chemical AFM-fishing (AFM-IF ) has been developed for the detection of proteins at ultra-low concentrations in solution. Using this method, a very low concentration of horseradish peroxidase (HRP) protein (10 17 M) was detected in solution. A theoretical model that allows the description of obtained experimental data, is proposed. This model takes into consideration both the transport of the protein from the bulk solution onto the AFM-chip surface and its irreversible binding to the activated area.
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Reference: Ivanov Yu.D., Danichev V.V., Pleshakova T.O., Shumov I.D., Ziborov V.S., Krokhin N.V., Zagumenniy M.N., Ustinov V.S., Smirnov L.P., Shironin A.V., Archakov A.I., Irreversible chemical afm-fishing for the detection of low-copied proteins, Biomeditsinskaya khimiya, 2014, vol: 60(1), 28-50.
This paper is also available as the English translation:10.1134/S1990750813010071
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