HPLC analysis of dehydroepiandrosterone sulfate in serum with use of solid-phase extraction on hyper cross-linked polystyrene (purosep-200)

   


1. State Medical Academy
2. Zabaikalsky State University, Chita, Russia
Type: Experimental study
DOI: 10.18097/PBMC20146006651      UDK: 542,98: 615,322      PubMed Id: 25552504
Year: 2014 vol: 60  issue:6  pages: 651-654
Abstract: We have developed a simple HPLC method for analysis of the dehydroepiandrosterone sulfate (DHEA-sulfate) in serum with use a new procedure of solid-phase extraction (SPE) on hyper cross-linked polystyrene (Purosep-200) and fast chromatographic separation on the monolithic column under isocratic elution and UV detection at 200 nm. Complete SPE procedure lasts for about 7 min, chromatographic separation takes less than 6 min. Simplicity and high reproducibility of this method makes it attractive in routine clinical practice.
Download PDF:
Reference: Dutov A.A., Nikitin D.A., Lukyanova Yu.L., Sverkunova A.V., Martinova A.V., Ermolina A.V., HPLC analysis of dehydroepiandrosterone sulfate in serum with use of solid-phase extraction on hyper cross-linked polystyrene (purosep-200), Biomeditsinskaya khimiya, 2014, vol: 60(6), 651-654.
Bibliography
 2016(Vol:62)
 2015(Vol:61)
 2014(Vol:60)
 2013(Vol:59)
 2012(Vol:58)
 2011(Vol:57)
 2010(Vol:56)
 2009(Vol:55)
 2008(Vol:54)
 2007(Vol:53)
 2006(Vol:52)
 2005(Vol:51)
 2004(Vol:50)
 2003(Vol:49)