Abstract: A method of atomic force microscopy-based fishing (AFM fishing) has been developed for protein detection in the analyte solution using a chip with an immobilized aptamer. This method is based on the biospecific fishing of a target protein from a bulk solution onto the small AFM chip area with the immobilized aptamer to this protein used as the molecular probe. Such aptamer-based approach allows to increase an AFM image contrast compared to the antibody-based approach. Mass spectrometry analysis used after the biospecific fishing to identify the target protein on the AFM chip has proved complex formation. Use of the AFM chip with the immobilized aptamer avoids interference of the antibody and target protein peaks in a mass spectrum.
Reference: Bukharina N.S., Ivanov Yu.D., Pleshakova T.O., Frantsuzov P.A., Andreeva E.Yu., Kaysheva A.L., Izotov A.A., Pavlova T.I., Ziborov V.S., Radko S.P., Archakov A.I., Atomic force microscopy fishing of gp120 on immobilized aptamer and its mass spectrometry identification, Biomeditsinskaya khimiya, 2015, vol:
5. Rakhmetova S.Yu., Radko S.P., Gnedenko O.V., Bodoev N.V., Ivanov A.S., Archakov A.I., Photoaptamer heterodimeric constructs as a new approach to enhance the efficiency of formation of photocrosslinking with a target protein, Biomeditsinskaya khimiya, 2010, 56(1), 72-81. CrossRef Scholar google search
15. Barbas C.F., Björling E., Chiodi F., Dunlop N., Cababa D., Jones T.M., Zebedee S.L., Persson M.A., Nara P.L., Norrby E. (1992) Proc. Natl. Acad. Sci. USA, 89, 9339-9343. CrossRef Scholar google search