SOI-nanowire biosensor for the detection of D-NFAT 1 protein


1. Institute of Biomedical Chemistry, Moscow, Russia
2. Rzhanov Institute of Semiconductor Physics, Novosibirsk, Russia
Type: Experimental/clinical study
DOI: 10.18097/PBMC20156104462      UDK: 557.152.3, 538.975, 621.382.3      PubMed Id: 26350736
Year: 2015 vol: 61  issue:4  pages: 462-467
Abstract: The nanowire (NW) detection is one of fast-acting and high-sensitive methods allowing to reveal potentially relevant protein molecules. A NW biosensor based on the silicon-on-insulator (SOI)-structures was used for biospecific label-free detection of NFAT 1 (D-NFAT 1) oncomarker in real time. For this purpose, SOI-nanowires (NWs) were modified with aptamers against NFAT 1 used as molecular probes. It was shown that using this biosensor it is possible to reach the sensitivity of ~10-15 M. This sensitivity was comparable with that of the NW biosensor with immobilized antibodies used as macromolecular probes. The results demonstrate promising approaches used to form the sensor elements for high-sensitive disease diagnostics
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Reference: Malsagova K.A., Ivanov Yu.D., Pleshakova T.O., Kozlov A.F., Krohin N.V., Kaysheva A.L., Shumov I.D., Popov V.P., Naumova O.V., Fomin B.I., Nasimov D.A., SOI-nanowire biosensor for the detection of D-NFAT 1 protein, Biomeditsinskaya khimiya, 2015, vol: 61(4), 462-467.
This paper is also available as the English translation:10.1134/S199075081403010X