Determination of glutamic acid in biological material by capillary electrophoresis


1. Southern federal university, Rostov-on-Don, Russia
2. Rostov Scientific Research Institute of Obstetrics and Pediatrics, Rostov-on-Don, Russia
Type: Experimental study
DOI: 10.18097/PBMC20156105628      UDK: 543.545.618.346-008.8      PubMed Id: 26539871
Year: 2015 vol: 61  issue:5  pages: 628-631
Abstract: The conditions for the identification and determination of Glutamic acid by capillary zone electrophoresis without their preliminary derivatization have been optimized. The effect of concentration of buffer electrolyte and pH on determination of Glutamic acid has been investigated. It is shown that the 5 Mm borate buffer concentration and a pH 9.15 are optimal. Quantitative determination of glutamic acid has been carried out using a linear dependence between the concentration of the analyte and the area of the peak. The accuracy and reproducibility of the determination are confirmed by the method “introduced - found”. Glutamic acid has been determined in the placenta homogenate. The duration of analysis doesn’t exceed 30 minutes. The results showed a decrease in the level of glutamic acid in cases of pregnancy complicated by placental insufficiency compared with the physiological, and this fact allows to consider the level of glutamic acid as a possible marker of complicated pregnancy.
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Reference: Narezhnaya E., Krukier I., Avrutskaya V., Degtyareva A., Igumnova E.A., Determination of glutamic acid in biological material by capillary electrophoresis, Biomeditsinskaya khimiya, 2015, vol: 61(5), 628-631.
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