1. Federal Research and Clinical Center of Physical-Chemical Medicine, Moscow, Russia; Moscow Institute of Physics and Technology, Dolgoprudniy, Russia 2. Federal Research and Clinical Center of Physical-Chemical Medicine, Moscow, Russia 3. Moscow Institute of Physics and Technology, Dolgoprudniy, Russia 4. Department of Urology, Moscow State Medical Stomatological University, Moscow, Russia
Abstract: The RNA-seq approach for prostate cancer candidate RNA biomarkers screening in plasma and urine obtained by minimally invasive or noninvasive methods is proved to be feasible. Significant amount of RNA biomarkers associated with prostate cancer according to the literature were found in plasma and urine samples obtained from patients with benign prostatic hyperplasia (BPH). The number of detected markers was shown to vary in accordance with method of library preparation used for transcriptome profiling. The detection of known RNA biomarkers for prostate cancer in urine and plasma samples shows the feasibility of such method for minimally invasive diagnostics. The fact of presence of the same RNA biomarkers in samples from patients with BPH suggests their possible lack of specificity and confirms the need for further research in this area.