Oxidative modification of glyceraldehyde-3-phosphate dehydrogenase influences its interaction with endogenous neuroprotector isatin

   


1. Institute of Biomedical Chemistry, Moscow, Russia
2. Moscow State University, Moscow, Russia
Type: Experimental study
DOI: 10.18097/PBMC20166202160      UDK: 577.174.5      PubMed Id: 27143373
Year: 2016 vol: 62  issue:2  pages: 160-163
Abstract: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a classical glycolytic redox sensitive enzyme, exhibits various non-glycolytic functions, which are considered to be especially important for progression of various neurodegenerative diseases. GAPDH binds isatin (indole-dione-2,3), an endogenous indole often used as a parent component in numerous derivatives demonstrating diverse pharmacological (including neuroprotector) activities. In this study we have investigated binding of intact and mildly oxidized GAPDH to immobilized isatin, using an optical biosensor technique, employing surface plasmon resonance (SPR), and the effect of isatin as a probe for this binding. Mild GAPDH oxidation by 70 mM H2O2 increased enzyme dissociation from immobilized isatin. Since GAPDH is considered as a putative target for various neuroprotector agents, this suggests that its redox state determines sensitivity to neuroprotective agents, and oxidative stress typical for various neurodegenerative disorders may significantly reduce pharmacological effectiveness of such compounds
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Reference: Buneeva O.A., Gnedenko O.V., Medvedeva M.V., Ivanov A.S., Medvedev A.E., Oxidative modification of glyceraldehyde-3-phosphate dehydrogenase influences its interaction with endogenous neuroprotector isatin, Biomeditsinskaya khimiya, 2016, vol: 62(2), 160-163.
This paper is also available as the English translation:10.1134/S1990750815020043
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