Antibodies against modified low-density lipoproteins and their complexes in blood of patients with various manifestations of atherosclerosis

   


1. Institute of Experimental Medicine, Saint Petersburg, Russia
2. Institute of Experimental Medicine, Saint Petersburg, Russia; Saint-Petersburg State University, Saint Petersburg, Russia
Type: Clinical and diagnostic research
DOI: 10.18097/PBMC20166204471      UDK: 571.27      PubMed Id: 27563003
Year: 2016 vol: 62  issue:4  pages: 471-475
Abstract: The study included 79 patients with coronary artery disease, 25 individuals with preclinical atherosclerosis and 59 healthy controls. Key lipid parameters were examined in all the participants. Levels of antibodies (Abs) against (IgG and IgM) LDL modified by malondialdehyde (MDA), acetic anhydride and hypochlorite, were determined by the enzyme-linked immunosorbent assay (ELISA). Abs specificity was tested by competitive ELISA. Circulating immune complexes (CIC) were isolated by precipitation in polyethylene glycol. Abs to hypochlorite-modified low density lipoprotein (hypochlorite-LDL) were detected in the serum samples. These Abs did not demonstrate cross-reactivity with MDA-modified LDL (MDA-LDL) and acetylated LDL (acetyl-LDL). Patients with coronary artery disease had increased levels of CIC (p<0.0001) and decreased levels of Abs (IgM) to hypochlorite-LDL, compared with healthy controls and patients with preclinical atherosclerosis (p=0.006). A correlation between the levels of Abs (IgG) to the hypochlorite-LDL and Abs to MDA- and acetyl-LDL was found. There was a correlation between the content of the Abs (IgM) to MDA- and acetyl-LDL and the concentration of CIC-cholesterol. Lipid parameters did not correlate with Abs levels.
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Reference: Belik I.V., Ivantsova A.A., Mamedova Z.E., Denisenko A.D., Antibodies against modified low-density lipoproteins and their complexes in blood of patients with various manifestations of atherosclerosis, Biomeditsinskaya khimiya, 2016, vol: 62(4), 471-475.
This paper is also available as the English translation:10.1134/S1990750816040028
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