Multiomics study of HepG2 cell line proteome


1. Institute of Biomedical Chemistry, Moscow, Russia
Type: OMICS-technologies
DOI: 10.18097/PBMC20176305373      PubMed Id: 29080867
Year: 2017 vol: 63  issue:5  pages: 373-378
Abstract: Current proteomic studies are generally focused on the most abundant proteoforms encoded by canonical nucleic sequences. Transcriptomic and proteomic data, accumulated in a variety of postgenome sources and coupled with state-of-art analytical technologies, allow to start the identification of aberrant (non-canonical) proteoforms. The main sources of aberrant proteoforms are alternative splicing, single nucleotide polymorphism, and post-translational modifications. The aim of this work was to estimate the heterogeneity of HepG2 proteome. We suggested multiomics approach, which combines transcriptomic (RNAseq) and proteomic (2DE-MS/MS) methods, as a promising strategy to explore the proteome.
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Reference: Poverennaya E.V., Kiseleva O.I., Ponomarenko E.A., Naryzhny S.N., Zgoda V.G., Lisitsa A.V., Multiomics study of HepG2 cell line proteome, Biomeditsinskaya khimiya, 2017, vol: 63(5), 373-378.
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