Possible use of the growing liver biological set for hepatic recovery after toxic damage (an experimental study)


1. Sechenov First Moscow State Medical University, Moscow, Russia;
2. NRC Institute of Immunology, Moscow, Russia
3. Institute of Organic Chemistry of the Russian Academy of Sciences, Moscow, Russia
4. Institute of General Pathology and Pathophysiology, Moscow, Russia
Type: Technologies of precision medicine
DOI: 10.18097/PBMC20176305440      PubMed Id: 29080878
Year: 2017 vol: 63  issue:5  pages: 440-446
Abstract: The lack of acceptable pharmacological approaches for restoration of the injured liver is associated with complex of mechanisms involved in hepatic regeneration and with difficulty of the target selection. The aim of this research was to study the hepatoprotective function of the extract from both the growing and regenerating liver containing a natural set of factors crucial for the hepatic restoration. Extracts from both regenerating liver of rats after 70% hepatic resection and the growing liver of neonatal pigs were obtained using own original technique. The set of resultant extracts was named as the hepatic regeneration set (HRS). HRS fractionation was carried out using the Toyopearl HW-50S sorbent. The efficiency of HRS and its fractions was estimated using a model of the mouse liver thioacetamide injury and monitoring hepatic enzyme activity in blood serum. The activities of AST and ALT in intact animals were 50 U/l and 80 U/l, respectively; after thioacetamide administration they increased to 2059±212 U/l and 4280±440 E/l, respectively (p<0.05). Treatment of injured animals with HRS from the rat regenerating liver resulted in a significant decrease of transaminase activities to 924±148 U/l (AST; p<0.05) and 1633±308 U/l (ALT; p<0.05). A similar effect was observed after treatment with HRS from the neonatal pig liver: the AST decreased to 937±138 U/l (p<0.05), while ALT activity decreased to 1710±237 U/l (p<0.05). HRs fractionation resulted in identification two active fractions characterized by much higher (8-29) hepatotropic effect that that of the whole extract. These fractions contained peptide/protein components with the range of molecular mass of 3-60 kDa (fraction 1) and 3-25 kDa (fraction 2a). Fraction 1 also contained some polynucleotides in fraction 1. Subsequent studies of these fractions exceeding the hepatotropic effect of original HRS is clearly needed to identify their individual components by immunochromatography methods, ELISA, MRM mass spectrometry and quantitative PCR.
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Reference: Gal`perin E.I., Ataullakhanov R.I., Dyuzheva T.G., Platonova L.V., Melnikova T.M., Monakov M.Yu., Dudchenko A.M., Lyundup A.V., Klabukov I.D., Possible use of the growing liver biological set for hepatic recovery after toxic damage (an experimental study), Biomeditsinskaya khimiya, 2017, vol: 63(5), 440-446.