Changes in laboratory parameters of peripheral blood reflect cell and protein content of the immune system in bone resorption

   


1. Ural Research Institute of Phthisiopulmonology, Yekaterinburg, Russia; Ural State Medical University, Yekaterinburg, Russia
2. Chaklin Ural Institute of Traumatology and Orthopedics, Yekaterinburg, Russia; Ural State Medical University, Yekaterinburg, Russia
Type: Technologies of precision medicine
DOI: 10.18097/PBMC20176305447      PubMed Id: 29080879
Year: 2017 vol: 63  issue:5  pages: 447-452
Abstract: The aim of this study was to investigate dynamic changes in the laboratory parameters of peripheral blood, reflecting cellular and protein composition of the immune system in bone resorption. The study involved 108 patients with degenerative-dystrophic and posttraumatic disorders of the hip joint before and after joint replacement surgery. Half of the cases resulted in bone resorption. Dynamic monitoring was performed up to 7.5 years. It included flow cytometry (Coulter Epics XL, USA) and enzyme immunoassay to determine the amount of immunocompetent cells, immunoglobulin class M, A, G, E, cytokines, acute phase proteins (C-reactive protein, fibrinogen, albumin, ceruloplasmin, haptoglobin), parameters of neutrophil functional activity (lactoferrin cationic protein, myeloperoxidase, superoxide anion production). The results of the study revealed the leading role of haptoglobin, albumin, and IL-1b in bone resorption. The use of multiple regression analysis made it possible to propose criteria for prediction of bone resorption. In particular, the Ig G concentration one month after operation at a value of 13 or less g/l with a probability of 86.8% suggests a high risk of bone tissue destruction in the operated area of the joint (diagnostic sensitivity and diagnostic specificity of 85.7% and 86.9%, respectively). Determination of the IL-1b level also has a good predictive power: its concentration exceeding 191.2 pg/ml six month after surgery with the probability of 87.4% suggests destruction of bone tissue in the operated area of the joint (diagnostic sensitivity and diagnostic specificity of 87.2% and 88.1%, respectively).
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Reference: Berdugina O.V., Berdugin K.A., Changes in laboratory parameters of peripheral blood reflect cell and protein content of the immune system in bone resorption, Biomeditsinskaya khimiya, 2017, vol: 63(5), 447-452.
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