Quality control study of engineered skin tissue


1. Institute of Biomedical Chemistry, Moscow, Russia
2. Research and Manufacturing Association “Perspectiva”, Novosibirsk, Russia
Type: Experimental study
DOI: 10.18097/PBMC20186401010      PubMed Id: 29460829
Year: 2018 vol: 64  issue:1  pages: 10-15
Abstract: OMERO service was used to annotate the cell line HaCaT microscope images by two independent expert groups. The images were obtained in the course of developing tissue-engineered epithelium which consisted of several layers of the keratinocytes. Evaluation of expert opinions was performed by calculation of specificity, sensitivity and accuracy. The best convergence of opinions (91%) was achieved for the confluence of the cell monolayers. Accuracy 70% was observed in determining the extent of cell differentiation after 10 days of incubation. The paper illustrates the usefulness of OMERO service for dynamic cross-validation of quality in the development and standardization of cell preparations.
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Reference: Nakhod K.V., Rusanov A.L., Luzgina E.D., Druzhilovskiy D.S., Luzgina N.G., Lisitsa A.V., Quality control study of engineered skin tissue, Biomeditsinskaya khimiya, 2018, vol: 64(1), 10-15.
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