Methods for determining of cytochrome P450 isozymes functional activity

   


1. Institute of Biomedical Chemistry, Moscow, Russia; Pirogov Russian National Research Medical University (RNRMU), Moscow, Russia
Type: Review
DOI: 10.18097/PBMC20186402149     
Year: 2018 vol: 64  issue:2  pages: 149-168
Abstract: The review is dedicated to modern methods and technologies for determining of cytochrome P450 isozymes functional activity, such as absorbance and fluorescent spectroscopy, electron paramagnetic resonance (EPR), nuclear magnetic resonance (NMR), Raman, Mossbauer, and X-ray spectroscopy, surface plasmon resonance (SPR), atomic force microscopy (AFM). Methods of molecular genetic analysis were reviewed from personalized medicine point of view. The use of chromate-mass-spectrometric methods for cytochrome P450-dependent catalytic reactions' products was discussed. The review covers modern electrochemical systems based on cytochrome P450 isozymes for their catalytic activity analysis, their use in practice and further development perspectives for experimental pharmacology, biotechnology and translational medicine.
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Reference: Kuzikov A.V., Masamrekh R.A., Archakov A.I., Shumyantseva V.V., Methods for determining of cytochrome P450 isozymes functional activity, Biomeditsinskaya khimiya, 2018, vol: 64(2), 149-168.
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