Comparative proteomic profiling of nuclear and cytosolic fractions from cell lines of different origin

   


1. Institute of Biomedical Chemistry, Moscow, Russia
2. Institute of Biomedical Chemistry, Moscow, Russia; National Research University Higher School of Economics, Moscow, Russia
Type: Experimental study
DOI: 10.18097/PBMC20186403233      PubMed Id: 29964258
Year: 2018 vol: 64  issue:3  pages: 233-240
Abstract: Proteomic analysis of the nuclear fraction is of great importance, since many cellular processes are initiated in the nucleus. Refinement and choice of experimental procedures for cell lysate fractionation and parameters for mass spectrometric detection and data processing continue to be of current interest. The mass spectrometry analysis presented here was tested on human cell lines derived from different tissues: HL-60 (peripheral blood); HepG2 (liver); EA.hy926 (vascular endothelium). High reproducibility of results and their consistency with biological properties of the objects under study were demonstrated. The use of cells of different types made it possible to reveal a set of 16 proteins whose LFQ-values allow for the discrimination between proteome fractions regardless of cell origin. Also, a set of 16 proteins is suggested which are associated with individual characteristics of cell lines regardless of cell fraction. These protein panels can serve as parameters to verify the proteomic analysis done was of sufficient quality, in particular as indicators of successful fractionation of cell or tissue lysate.
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Reference: Vakhrushev I.V., Novikova S.E., Tsvetkova A.V., Pyatnitskiy M.A., Yarygin K.N., Comparative proteomic profiling of nuclear and cytosolic fractions from cell lines of different origin, Biomeditsinskaya khimiya, 2018, vol: 64(3), 233-240.
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