Исследовано влияние модуляторов VDAC-каналов — G3139 и эрастина — на функционирование митохондриальной поры (mitochondrial permeability transition pore, мPTP), а также на изменения в содержании белков, регулирующих работу мPTP (VDAC, CNPаза и TSPO) в митохондриях печени крыс с хронической алкогольной интоксикацией. Показано, что митохондрии печени крыс, получавших этанол, более чувствительны к индукции мPTP. Более того, под воздействием этанола происходят изменения в экспрессии белков-регуляторов мPTP. G3139 и эрастин также способны влиять на исследуемые митохондриальные параметры, причём их действие в митохондриях печени крыс, получавших этанол, более выражено по сравнению с митохондриями печени контрольных крыс. Мы предполагаем, что результаты этого исследования могут помочь выяснить механизмы хронического действия этанола на митохондрии и внести вклад в разработку новых терапевтических стратегий для лечения патологий, связанных с потреблением этанола.
Бабурина Ю.Л., Звягина А.И., Одинокова И.В., Крестинина О.В. (2023) Влияние эрастина и G3139 на митохондрии печени крыс при хронической алкогольной интоксикации. Биомедицинская химия, 69(1), 62-71.
Бабурина Ю.Л. и др. Влияние эрастина и G3139 на митохондрии печени крыс при хронической алкогольной интоксикации // Биомедицинская химия. - 2023. - Т. 69. -N 1. - С. 62-71.
Бабурина Ю.Л. и др., "Влияние эрастина и G3139 на митохондрии печени крыс при хронической алкогольной интоксикации." Биомедицинская химия 69.1 (2023): 62-71.
Бабурина, Ю. Л., Звягина, А. И., Одинокова, И. В., Крестинина, О. В. (2023). Влияние эрастина и G3139 на митохондрии печени крыс при хронической алкогольной интоксикации. Биомедицинская химия, 69(1), 62-71.
Список литературы
Khambu B., Wang L., Zhang H., Yin X.M. (2017) The activation and function of autophagy in alcoholic liver disease. Curr. Mol. Pharmacol., 10(3), 165-171. CrossRef Scholar google search
Bonet-Ponce L., Saez-Atienzar S., da Casa C., Flores-Bellver M., Barcia J.M., Sancho-Pelluz J., Romero F.J., Jordan J., Galindo M.F. (2015) On the mechanism underlying ethanol-induced mitochondrial dynamic disruption and autophagy response. Biochim. Biophys. Acta, 1852(7), 1400-1409. CrossRef Scholar google search
Manzo-Avalos S., Saavedra-Molina A. (2010) Cellular and mitochondrial effects of alcohol consumption. Int. J. Environ. Res. Public Health, 7(12), 4281-4304. CrossRef Scholar google search
Samuvel D.J., Li L., Krishnasamy Y., Gooz M., Takemoto K., Woster P.M., Lemasters J.J., Zhong Z. (2022) Mitochondrial depolarization after acute ethanol treatment drives mitophagy in living mice. Autophagy, 18(11), 2671-2685. CrossRef Scholar google search
Rostovtseva T.K., Sheldon K.L., Hassanzadeh E., Monge C., Saks V., Bezrukov S.M., Sackett D.L. (2008) Tubulin binding blocks mitochondrial voltage-dependent anion channel and regulates respiration. Proc. Natl. Acad. Sci. USA, 105(48), 18746-18751. CrossRef Scholar google search
Shoshan-Barmatz V., Ben-Hail D. (2012) VDAC, a multi-functional mitochondrial protein as a pharmacological target. Mitochondrion, 12(1), 24-34. CrossRef Scholar google search
Pediaditakis P., Kim J.S., He L., Zhang X., Graves L.M., Lemasters J.J. (2010) Inhibition of the mitochondrial permeability transition by protein kinase A in rat liver mitochondria and hepatocytes. Biochem. J., 431(3), 411-421. CrossRef Scholar google search
Camara A.K.S., Zhou Y., Wen P.C., Tajkhorshid E., Kwok W.M. (2017) Mitochondrial VDAC1: Akey gatekeeper as potential therapeutic target. Front. Physiol., 8, 460. CrossRef Scholar google search
Shoshan-Barmatz V., De S., Meir A. (2017) The mitochondrial voltage-dependent anion channel 1, Ca2+ transport, apoptosis, and their regulation. Front. Oncol., 7, 60. CrossRef Scholar google search
Holmuhamedov E., Lemasters J.J. (2009) Ethanol exposure decreases mitochondrial outer membrane permeability in cultured rat hepatocytes. Arch. Biochem. Biophys., 481(2), 226-233. CrossRef Scholar google search
Lemasters J.J., Holmuhamedov E.L., Czerny C., Zhong Z., Maldonado E.N. (2012) Regulation of mitochondrial function by voltage dependent anion channels in ethanol metabolism and the warburg effect. Biochim. Biophys. Acta, 1818(6), 1536-1544. CrossRef Scholar google search
Kim J., Gupta R., Blanco L.P., Yang S., Shteinfer-Kuzmine A., Wang K., Zhu J., Yoon H.E., Wang X., Kerkhofs M., Kang H., Brown A.L., Park S.-J. et al. (2019) VDAC oligomers form mitochondrial pores to release mtDNA fragments and promote lupus-like disease. Science, 366(6472), 1531-1536. CrossRef Scholar google search
Navaneetha Krishnan S., Rosales J.L., Lee K.Y. (2020) mPTP opening caused by Cdk5 loss is due to increased mitochondrial Ca2+ uptake. Oncogene, 39(13), 2797-2806. CrossRef Scholar google search
Baburina Y., Odinokova I., Krestinina O. (2020) The effects of PK11195 and protoporphyrin IX can modulate chronic alcohol intoxication in rat liver mitochondria under the opening of the mitochondrial permeability transition pore. Cells, 9(8), DOI: 10.3390/cells9081774. CrossRef Scholar google search
King A.L., Swain T.M., Mao Z., Udoh U.S., Oliva C.R., Betancourt A.M., Griguer C.E., Crowe D.R., Lesort M., Bailey S.M. (2014) Involvement of the mitochondrial permeability transition pore in chronic ethanol-mediated liver injury in mice. Am. J. Physiol. Gastrointest. Liver. Physiol., 306(4), G265-G277. CrossRef Scholar google search
Lamarche F., Carcenac C., Gonthier B., Cottet-Rousselle C., Chauvin C., Barret L., Leverve X., Savasta M., Fontaine E. (2013) Mitochondrial permeability transition pore inhibitors prevent ethanol-induced neuronal death in mice. Chem. Res. Toxicol., 26(1), 78-88. CrossRef Scholar google search
Lieber C.S., de Carli L.M. (1989) Liquid diet technique of ethanol administration: 1989 update. Alcohol Alcohol., 24(3), 197-211. Scholar google search
ImageJ. Retrived January 20, 2023 from https://imagej.nih.gov/ij/. Scholar google search
Azarashvili T., Grachev D., Krestinina O., Evtodienko Y., Yurkov I., Papadopoulos V., Reiser G. (2007) The peripheral-type benzodiazepine receptor is involved in control of Ca2+-induced permeability transition pore opening in rat brain mitochondria. Cell Calcium, 42(1), 27-39. CrossRef Scholar google search
Reiser G., Kunzelmann U., Steinhilber G., Binmoller F.J. (1994) Generation of a monoclonal antibody against the myelin protein CNP (2′,3′-cyclic nucleotide 3′-phosphodiesterase) suitable for biochemical and for immunohistochemical investigations of CNP. Neurochem. Res., 19(12), 1479-1485. Scholar google search
Han J., Lee C., Hur J., Jung Y. (2022) Current therapeutic options and potential of mesenchymal stem cell therapy for alcoholic liver disease. Cells, 12(1), 22. CrossRef Scholar google search
Cederbaum A.I., Lu Y., Wu D. (2009) Role of oxidative stress in alcohol-induced liver injury. Arch. Toxicol., 83(6), 519-548. CrossRef Scholar google search
Mantena S.K., King A.L., Andringa K.K., Eccleston H.B., Bailey S.M. (2008) Mitochondrial dysfunction and oxidative stress in the pathogenesis of alcohol- and obesity-induced fatty liver diseases. Free Radic. Biol. Med., 44(7), 1259-1272. CrossRef Scholar google search
Briston T., Selwood D.L., Szabadkai G., Duchen M.R. (2019) Mitochondrial permeability transition: A molecular lesion with multiple drug targets. Trends Pharmacol. Sci., 40(1), 50-70. CrossRef Scholar google search
Federico M., de la Fuente S., Palomeque J., Sheu S.S. (2021) The role of mitochondria in metabolic disease: A special emphasis on heart dysfunction. J. Physiol., 599(14), 3477-3493. CrossRef Scholar google search
Rottenberg H., Hoek J.B. (2021) The mitochondrial permeability transition: Nexus of aging, disease and longevity. Cells, 10(1), 79. CrossRef Scholar google search
Baburina Y., Odinokova I., Krestinina O. (2021) Carbenoxolon is capable to regulate the mitochondrial permeability transition pore opening in chronic alcohol intoxication. Int. J. Mol. Sci., 22(19), 10249. CrossRef Scholar google search
Krestinina O., Odinokova I., Sotnikova L., Krestinin R., Zvyagina A., Baburina Y. (2022) Astaxanthin is able to prevent alcohol-induced dysfunction of liver mitochondria. Antioxidants (Basel), 11(10), 2019. CrossRef Scholar google search
Baines C.P., Kaiser R.A., Sheiko T., Craigen W.J., Molkentin J.D. (2007) Voltage-dependent anion channels are dispensable for mitochondrial-dependent cell death. Nat. Cell. Biol., 9(5), 550-555. Scholar google search
Zhou H., Hu S., Jin Q., Shi C., Zhang Y., Zhu P., Ma Q., Tian F., Chen Y. (2017) Mff-dependent mitochondrial fission contributes to the pathogenesis of cardiac microvasculature ischemia/reperfusion injury via induction of mROS-mediated cardiolipin oxidation and HK2/VDAC1 disassociation-involved mPTP opening. J. Am. Heart Assoc., 6(3), e005328. CrossRef Scholar google search
Chaudhuri A.D., Choi D.C., Kabaria S., Tran A., Junn E. (2016) Microrna-7 regulates the function of mitochondrial permeability transition pore by targeting VDAC1 expression. J. Biol. Chem., 291(12), 6483-6493. CrossRef Scholar google search
Yagoda N., von Rechenberg M., Zaganjor E., Bauer A.J., Yang W.S., Fridman D.J., Wolpaw A.J., Smukste I., Peltier J.M., Boniface J.J., Smith R., Lessnick S.L., Sahasrabudhe S., Stockwell B.R. (2007) RAS-RAF-MEK-dependent oxidative cell death involving voltage-dependent anion channels. Nature, 447(7146), 864-868. CrossRef Scholar google search
Bauer A.J., Gieschler S., Lemberg K.M., McDermott A.E., Stockwell B.R. (2011) Functional model of metabolite gating by human voltage-dependent anion channel 2. Biochemistry, 50(17), 3408-3410. CrossRef Scholar google search
Tan W., Loke Y.H., Stein C.A., Miller P., Colombini M. (2007) Phosphorothioate oligonucleotides block the VDAC channel. Biophys. J., 93(4), 1184-1191. CrossRef Scholar google search
Azarashvili T., Krestinina O., Baburina Y., Odinokova I., Grachev D., Papadopoulos V., Akatov V., Lemasters J.J., Reiser G. (2015) Combined effect of G3139 and TSPO ligands on Ca2+-induced permeability transition in rat brain mitochondria. Arch. Biochem. Biophys., 587, 70-77. CrossRef Scholar google search
Guo Y., Sun Z., Wang L., Jiang R., Shu Q., Xu G. (2022) Increased expression of TSPO-VDAC complex is correlated with NLRP3 inflammasome activation in diabetic retinopathy. Mol. Med. Rep., 26(6), 353. CrossRef Scholar google search
Hiser C., Montgomery B.L., Ferguson-Miller S. (2021) TSPO protein binding partners in bacteria, animals, and plants. J. Bioenerg. Biomembr., 53(4), 463-487. CrossRef Scholar google search
Azarashvili T., Krestinina O., Galvita A., Grachev D., Baburina Y., Stricker R., Evtodienko Y., Reiser G. (2009) Ca2+-dependent permeability transition regulation in rat brain mitochondria by 2′,3′-cyclic nucleotides and 2′,3′-cyclic nucleotide 3′-phosphodiesterase. Am. J. Physiol. Cell Physiol., 296(6), C1428-C1439. Scholar google search
Baburina Y., Azarashvili T., Grachev D., Krestinina O., Galvita A., Stricker R., Reiser G. (2015) Mitochondrial 2′,3′-cyclic nucleotide 3′-phosphodiesterase (CNP) interacts with mPTP modulators and functional complexes (I-V) coupled with release of apoptotic factors. Neurochem. Int., 90, 46-55. CrossRef Scholar google search
He Y., Wang W., Yang T., Thomas E.R., Dai R., Li X. (2022) The potential role of voltage-dependent anion channel in the treatment of Parkinson's disease. Oxid. Med. Cell. Longev., 2022, 4665530. CrossRef Scholar google search
Klapper-Goldstein H., Verma A., Elyagon S., Gillis R., Murninkas M., Pittala S., Paul A., Shoshan-Barmatz V., Etzion Y. (2020) VDAC1 in the diseased myocardium and the effect of VDAC1-interacting compound on atrial fibrosis induced by hyperaldosteronism. Sci. Rep., 10(1), 22101. CrossRef Scholar google search
