Сравнительный протеомный анализ почечной ткани нормотензивных (WKY) и гипертензивных (SHR) крыс выявил количественные и качественные изменения ряда белков. Специфичные для животных линии WKY (артериальное давление 110–120 мм рт. ст.) белки почек варьировали в диапазоне 13–16, специфичные для SHR (артериальное давление 180 мм рт. ст. и более) — в диапазоне 24–28, а общее число идентифицированных для обеих линий белков составило 972–975. При попарном сравнении всех возможных (SHR-WKY) вариантов идентифицированы 8 белков, специфичных только для нормотензивных животных, и 7 — только для гипертензивных. С учётом их биологической роли, отсутствие одних белков-ферментов у крыс-гипертоников (например, биливердинредуктаза А) снижает выработку молекул, проявляющих гипотензивные свойства, а появление других (бетаин-гомоцистеин S-метилтрансфераза 2, септин 2 и др.) может быть интерпретировано как компенсаторная реакция. На долю белков, относительное содержание которых менялось в почках не менее 2,5 раз, пришлось не более 5% всех идентифицированных белков. Среди белков, относительное содержание которых увеличивалось у гипертонических животных, наибольшую группу составляли белки, участвующие в процессах генерации энергии и углеводного обмена, а также антиоксидантные и защитные белки. В контексте развития гипертонии выявленные изменения, по-видимому, могут рассматриваться как компенсаторные. Среди белков, относительное содержание которых у крыс-гипертоников снизилось наиболее сильно, драматическое снижение ацил-КоA-синтетазы среднецепочечных жирных кислот (ACSM3), по-видимому, вносит важный вклад в развитие почечной патологии у этих животных.
Бунеева О.А. и др. Сравнительный протеомный анализ почечной ткани нормотензивных и гипертензивных крыс // Биомедицинская химия. - 2024. - Т. 70. -N 2. - С. 89-98.
Бунеева О.А. и др., "Сравнительный протеомный анализ почечной ткани нормотензивных и гипертензивных крыс." Биомедицинская химия 70.2 (2024): 89-98.
Бунеева, О. А., Федченко, В. И., Калошина, С. А., Завьялова, М. Г., Згода, В. Г., Медведев, А. Е. (2024). Сравнительный протеомный анализ почечной ткани нормотензивных и гипертензивных крыс. Биомедицинская химия, 70(2), 89-98.
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