Реналаза (RNLS) — вовлечённый в регуляцию артериального давления белок, которому свойственны различные функции внутри и снаружи клеток. Двадцатичленный пептид RP220, соответствующий аминокислотной последовательности RNLS человека 220–239, воспроизводит ряд эффектов внеклеточной RNLS и может связываться со многими внутриклеточными белками почек. Последовательность RP220 содержит несколько участков расщепления внеклеточными протеазами, в том числе с образованием пептидов RP224-232 и RP233-239. Целью настоящей работы было протеомное профилирование ткани почек нормотензивных крыс Wistar Kyoto (WKY) и крыс со спонтанной гипертензией SHR (spontaneously hypertensive rats), полученных на основе WKY, с использованием потенциальных протеолитических фрагментов (RP224-232 и RP233-239) пептида RP220 в качестве аффинных лигандов, в сравнении с данными для исходного пептида RP220. Полученные результаты свидетельствуют о том, что относительное содержание связавшихся с RNLS-пептидами белков SHR крыс по сравнению с таковым у крыс WKY наиболее ярко изменяется у пептида RP224-232. Практически все эти белки, за редким исключением, ассоциированы с сердечно-сосудистой патологией, многие с гипертензией. Таким образом, протеолитический процессинг RP220 приводит не к инактивации данного пептида, но к изменению его лигандных/регуляторных свойств, а также репертуара потенциальных белков-партнёров и, следовательно, белок-белковых взаимодействий, которые могут иметь возможное фармакологическое применение.
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Ключевые слова: реналаза, пептиды реналазы RP220, RP224-232 и RP233-239, артериальная гипертензия, крысы WKY и SHR, протеомное профилирование ткани почек
Бунеева О.А. и др. Взаимодействие белков почек нормо- и гипертензивных крыс с фрагментами реналазного пептида RP220 // Биомедицинская химия. - 2025. - Т. 71. -N 2. - С. 103-115.
Бунеева О.А. и др., "Взаимодействие белков почек нормо- и гипертензивных крыс с фрагментами реналазного пептида RP220." Биомедицинская химия 71.2 (2025): 103-115.
Бунеева, О. А., Федченко, В. И., Калошина, С. А., Завьялова, М. Г., Згода, В. Г., Медведев, А. Е. (2025). Взаимодействие белков почек нормо- и гипертензивных крыс с фрагментами реналазного пептида RP220. Биомедицинская химия, 71(2), 103-115.
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