К настоящему времени накоплен большой объём данных о биологической активности малотоксичного природного гликозида — глицирризиновой кислоты (ГК), однако механизм действия данного соединения на молекулярном уровне до конца не изучен. Расширение знаний о спектре клеточных белковых мишеней ГК способствует пониманию новых особенностей фармакодинамики. Целью работы была экспериментальная идентификация тканеспецифического спектра белковых молекул, взаимодействующих с глицирризиновой кислотой в модельной системе. Образцы интактного лизата ткани печени крысы инкубировали с ковалентно иммобилизованной ГК на EAH-сефарозе 4B с последующей элюцией аффинно выделенных белковых молекул и их трипсинолизом. С помощью масс-спектрометрического анализа были идентифицированы 88 потенциальных белковых мишеней ГК. Дополнительно, по результатам гель-хроматографического разделения лизата ГК влияла на полуколичественное распределение белков Aldh6a1, Decr1 и Sod1 во фракциях. Молекулярный докинг в программе Flare™ использовали для моделирования комплексов ГК и белков, по результатам которого выбрали 5 белков (Acox2, Acr1c9, Maoa, Mat1a, Nalcn) образующих с ГК комплексы с наиболее благоприятными параметрами ΔG и Rank score. Больше половины (57%) аффинно выделенных белков участвуют в процессах базового клеточного метаболизма и биотрансформации эндогенных и экзогенных соединений. Систематизированы и сопоставлены данные об ассоциациях потенциальных белковых мишеней ГК с заболеваниями и разными типами биологической активности ГК.
Ершов П.В. и др. Глицирризиновая кислота: новые потенциальные белковые мишени // Биомедицинская химия. - 2025. - Т. 71. -N 4. - С. 270-282.
Ершов П.В. и др., "Глицирризиновая кислота: новые потенциальные белковые мишени." Биомедицинская химия 71.4 (2025): 270-282.
Ершов, П. В., Яблоков, Е. О., Калужский, Л. А., Мезенцев, Ю. В., Гнеденко, О. В., Константинов, М. А., Торопыгин, И. Ю., Иванов, А. С. (2025). Глицирризиновая кислота: новые потенциальные белковые мишени. Биомедицинская химия, 71(4), 270-282.
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