Одновременное ингибирование mTOR и STING как подход к снижению уровня альфа-синуклеина и лизосфинголипидов в первичной культуре макрофагов периферической крови человека и клеточной линии нейробластомы SH-SY5Y: перспективы терапии болезни Паркинсона
1. Петербургский институт ядерной физики имени Б.П. Константинова Научно-исследовательский центр “Курчатовский институт”, Гатчина, Россия; Первый Санкт-Петербургский государственный медицинский университет имени академика И.П. Павлова, Санкт-Петербург, Россия 2. Петербургский институт ядерной физики имени Б.П. Константинова Научно-исследовательский центр “Курчатовский институт”, Гатчина, Россия 3. Научно-исследовательский институт детской онкологии, гематологии и трансплантологии имени Р.М. Горбачевой, Санкт-Петербург, Россия 4. Медико-генетический научный центр имени академика Н.П. Бочкова, Москва, Россия
В первичной культуре макрофагов периферической крови здоровых доноров и клеточной линии нейробластомы SH-SY5Y исследовали сочетанное влияние разнонаправленных препаратов: ингибитора mTOR (Torin 1), ключевого регулятора аутофагии, и ингибитора STING (H-151), ключевого регулятора воспаления, на параметры аутофаголизосомной системы. Сочетанное применение этих препаратов приводило к снижению уровня лизосфинголипидов, являющихся триггерами олигомеризации альфа-синуклеина, а также к снижению уровня мономерного и нейротоксичного фосфорилированного (Ser129) альфа-синуклеина и увеличению уровня тирозингидроксилазы. Полученные результаты открывают новые перспективы для применения комбинированной терапии предложенных препаратов в лечения как заболеваний, связанных с дисфункцией лизосом, так и с нейродегенеративными патологиями.
Безрукова А.И., Башарова К.С., Галкина Е.С., Епифановская О.С., Байдакова Г.В., Захарова Е.Ю., Пчелина С.Н., Усенко Т.С. (2026) Одновременное ингибирование mTOR и STING как подход к снижению уровня альфа-синуклеина и лизосфинголипидов в первичной культуре макрофагов периферической крови человека и клеточной линии нейробластомы SH-SY5Y: перспективы терапии болезни Паркинсона. Биомедицинская химия, 72(1), 42-61.
Безрукова А.И. и др. Одновременное ингибирование mTOR и STING как подход к снижению уровня альфа-синуклеина и лизосфинголипидов в первичной культуре макрофагов периферической крови человека и клеточной линии нейробластомы SH-SY5Y: перспективы терапии болезни Паркинсона // Биомедицинская химия. - 2026. - Т. 72. -N 1. - С. 42-61.
Безрукова А.И. и др., "Одновременное ингибирование mTOR и STING как подход к снижению уровня альфа-синуклеина и лизосфинголипидов в первичной культуре макрофагов периферической крови человека и клеточной линии нейробластомы SH-SY5Y: перспективы терапии болезни Паркинсона." Биомедицинская химия 72.1 (2026): 42-61.
Безрукова, А. И., Башарова, К. С., Галкина, Е. С., Епифановская, О. С., Байдакова, Г. В., Захарова, Е. Ю., Пчелина, С. Н., Усенко, Т. С. (2026). Одновременное ингибирование mTOR и STING как подход к снижению уровня альфа-синуклеина и лизосфинголипидов в первичной культуре макрофагов периферической крови человека и клеточной линии нейробластомы SH-SY5Y: перспективы терапии болезни Паркинсона. Биомедицинская химия, 72(1), 42-61.
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