The method of estimation of lysozyme activity in serum when chitin azure is used as substrate is described. The basis of incubation medium is 0,1 M acetate buffer, pH 5,0. It was judged about activity of lysozyme by fluorescence of azure in incubation medium at the end of three hour incubation at 37°C after separation of non-reacted chitin azure by short time centrifugation. It was shown that development of wound infection is accompanied by increasing of chitinase activity of lysozyme in serum.
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Keywords: lysozyme, serum, chitin azure, method of determination
Citation:
Pisarzhevskií S.A., Globa A.G., Shirshov O.N., Marchuk A.I., Svetuhin A.M., Karelin A.A. (1997) The method of estimation of lysozyme activity in serum with chitin azure as substrate. Voprosy Meditsinskoi Khimii, 43(3), 185-190.
Pisarzhevskií S.A. et al. The method of estimation of lysozyme activity in serum with chitin azure as substrate // Voprosy Meditsinskoi Khimii. - 1997. - V. 43. -N 3. - P. 185-190.
Pisarzhevskií S.A. et al., "The method of estimation of lysozyme activity in serum with chitin azure as substrate." Voprosy Meditsinskoi Khimii 43.3 (1997): 185-190.
Pisarzhevskií, S. A., Globa, A. G., Shirshov, O. N., Marchuk, A. I., Svetuhin, A. M., Karelin, A. A. (1997). The method of estimation of lysozyme activity in serum with chitin azure as substrate. Voprosy Meditsinskoi Khimii, 43(3), 185-190.
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