Isatin (indole-2,3-dione) is an endogenous indole found in the mammalian brain, peripheral organs and body fluids. It acts as a neuroprotector, which decreases manifestation of locomotor impairments in animal models of Parkinson's disease. A wide range of biological activity of isatin is associated with interaction of this regulator with numerous isatin-binding proteins. The aim of this study was to investigate the profile of brain isatin-binding proteins in mice with MPTP-induced Parkinsonism (90 min and seven days after administration of this neurotoxin). A single dose administration of MPTP (30 mg/kg, ip.) was accompanied by locomotor impairments in the open field test 90 min after administration; seven days after MPTP administration locomotor activity of mice significantly improved but did not reach the control level. Five independent experiments on proteomic profiling of isatin-binding proteins resulted in confident identification of 96±12 proteins. Development of MPTP-induced locomotor impairments was accompanied by a significant decrease in the number of isatin-binding proteins (63±6; n=5; p<0.01). Seven days after MPTP administration the total number of identified proteins increased and reached the control level (132±34; n=4). The profiles of isatin-binding proteins were rather specific for each group of mice: in the control group these proteins (which were not found in both groups of MPTP-treated mice) represented more than 70% of total proteins. In the case of MPTP treated mice this parameter was 60% (90 min after MPTP administration) and >82% (seven days after MPTP administration). The major changes were found in the groups of isatin-binding proteins involved into cytoskeleton formation and exocytosis, regulation of gene expression, cell division and differentiation and also proteins involved in signal transduction.
Buneeva O.A., Kopylov A.T., Nerobkova L.N., Kapitsa I.G., Zgoda V.G., Medvedev A.E. (2017) The effect of neurotoxin MPTP administration to mice on the proteomic profile of brain isatin-binding proteins. Biomeditsinskaya Khimiya, 63(4), 316-320.
Buneeva O.A. et al. The effect of neurotoxin MPTP administration to mice on the proteomic profile of brain isatin-binding proteins // Biomeditsinskaya Khimiya. - 2017. - V. 63. -N 4. - P. 316-320.
Buneeva O.A. et al., "The effect of neurotoxin MPTP administration to mice on the proteomic profile of brain isatin-binding proteins." Biomeditsinskaya Khimiya 63.4 (2017): 316-320.
Buneeva, O. A., Kopylov, A. T., Nerobkova, L. N., Kapitsa, I. G., Zgoda, V. G., Medvedev, A. E. (2017). The effect of neurotoxin MPTP administration to mice on the proteomic profile of brain isatin-binding proteins. Biomeditsinskaya Khimiya, 63(4), 316-320.
Hamaue N., Yamazaki N., Terado M., Minami M., Ohno K., Ide H., Ogata A., Honma S., Tashiro K. (2000) Res. Commun. Mol. Pathol. Pharmacol., 108, 63-73. Scholar google search
Crumeyrolle-Arias M., Buneeva O., Zgoda V., Kopylov A., Cardona A., Tournaire M.C., Pozdnev V., Glover V., Medvedev A. (2009) J. Neurosci. Res., 87, 2763-2772. CrossRef Scholar google search
Buneeva O., Gnedenko O., Zgoda V., Kopylov A., Glover V., Ivanov A., Medvedev A., Archakov A. (2010) Proteomics, 10, 23-37. CrossRef Scholar google search
Hamaue N., Minami M., Terado M., Hirafuji M., Endo T., Machida M., Hiroshige T., Ogata A., Tashiro K., Saito H., Parvez S.H. (2004) Neurotoxicology, 25, 205-213. CrossRef Scholar google search
Minami M., Hamaue N., Hirafuji M., Saito H., Hiroshige T., Ogata A., Tashiro K., Parvez S.H. (2006) J. Neural. Transm., Suppl. 71, 87-95. Scholar google search
