Renalase (RNLS) is a protein exhibiting various functions inside and outside cells. A series of synthetic peptides, corresponding to the fragments of the RNLS amino acid sequence, may reproduce the effects of the full-length RNLS. In this study we have investigated the interaction of proteins in the mitochondrial brain fraction of rats with the full-length RNLS and its peptides RP211-223 (peptide 1) and RP224-232 (peptide 2) immobilized on CNBr-activated Sepharose. Proteomic profiles of proteins bound to the full-length RNLS and its peptide RP211-223 were significantly different, whereas RP224-232 bound a small number of proteins (6), similar to those bound to the full-length RNLS. However, most proteins bound to RNLS and its peptides were multifunctional and associated with neurodegenerative pathology (Alzheimer’s and Parkinson’s diseases, etc.).
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Keywords: renalase, renalase peptides RP211-223 and RP224-232, proteomic profiling, rat brain mitochondria, neurodegeneration
Citation:
Buneeva O.A., Fedchenko V.I., Kaloshina S.A., Kaloshin A.A., Zgoda V.G., Medvedev A.E. (2026) Interaction of renalase and its peptides RP211-223 and RP224-232 with the mitochondrial fraction of rat brain: a link to neurodegeneration?. Biomeditsinskaya Khimiya, 72(3), 220-229.
Buneeva O.A. et al. Interaction of renalase and its peptides RP211-223 and RP224-232 with the mitochondrial fraction of rat brain: a link to neurodegeneration? // Biomeditsinskaya Khimiya. - 2026. - V. 72. -N 3. - P. 220-229.
Buneeva O.A. et al., "Interaction of renalase and its peptides RP211-223 and RP224-232 with the mitochondrial fraction of rat brain: a link to neurodegeneration?." Biomeditsinskaya Khimiya 72.3 (2026): 220-229.
Buneeva, O. A., Fedchenko, V. I., Kaloshina, S. A., Kaloshin, A. A., Zgoda, V. G., Medvedev, A. E. (2026). Interaction of renalase and its peptides RP211-223 and RP224-232 with the mitochondrial fraction of rat brain: a link to neurodegeneration?. Biomeditsinskaya Khimiya, 72(3), 220-229.
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