Изящество механизмов сплайсинга пре-мРНК не перестаёт интересовать ученых даже спустя более полувека с момента открытия того факта, что кодирующие участки в генах прерываются некодирующими. Превалирующее большинство генов человека имеют несколько вариантов мРНК, которые, в свою очередь, кодируют структурно и функционально разные варианты белков — в тканезависимой манере и с привязкой к конкретным этапам развития организма. Нарушение паттернов сплайсинга смещает баланс функционально различающихся белков в живой системе, искажает нормальные молекулярные пути и может спровоцировать возникновение и развитие патологий. За последние два десятилетия выполнено множество исследований в различных областях наук о жизни для более глубокого понимания механизмов сплайсинга и степени его влияния на функционирование живых систем. Целью данного обзора было суммирование экспериментальных и вычислительных подходов, используемых для выяснения функций сплайс-опосредованных белковых продуктов одного гена: на основе собственного опыта, накопленного в лаборатории интерактомики протеоформ Института биомедицинской химии, и лучших мировых практик.
Киселева О.И., Арзуманян В.А., Курбатов И.Ю., Поверенная Е.В. (2024) In silico и in cellulo подходы для функциональной аннотации сплайс-форм белков человека. Биомедицинская химия, 70(5), 315-328.
Киселева О.И. и др. In silico и in cellulo подходы для функциональной аннотации сплайс-форм белков человека // Биомедицинская химия. - 2024. - Т. 70. -N 5. - С. 315-328.
Киселева О.И. и др., "In silico и in cellulo подходы для функциональной аннотации сплайс-форм белков человека." Биомедицинская химия 70.5 (2024): 315-328.
Киселева, О. И., Арзуманян, В. А., Курбатов, И. Ю., Поверенная, Е. В. (2024). In silico и in cellulo подходы для функциональной аннотации сплайс-форм белков человека. Биомедицинская химия, 70(5), 315-328.
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