Doxorubicin-resistant MCF-7Dox line, which is a derivative of the drug-sensitive MCF-7 human breast carcinima line, differs from the latter by a strongly reduced expression of the α2β1 integrin and a highly increased expression of the α5β1 receptor. Silencing of this integrin in the MCF-7Dox cells by transfection with α5-specific siRNA markedly stimulated anoikis and increased sensitivity of the cells to doxorubicin. α5β1 silencing also leads to significant inhibition of the activity of kinases Akt and Erk2 in MCF-7Dox cells. Our results suggest that integrins α5β1-induced signals, controlling distinct aspects of cell behavior, are conducted through the common signal pathways.
Morozevich G.E. и др., Implication of integrin α5β1 in human breast carcinoma apoptosis and drug resistance //Biomeditsinskaya khimiya.- 2011. -Т.57. -№ 1. С. 77-84.
Morozevich G.E. и др., "Implication of integrin α5β1 in human breast carcinoma apoptosis and drug resistance." Biomeditsinskaya khimiya 57.1 (2011): 77-84.
Morozevich G.E., Kozlova N.I., Ushakova N.A., Preobrazhenskaya M.E., Berman A.E. (2011). Implication of integrin α5β1 in human breast carcinoma apoptosis and drug resistance. Biomeditsinskaya khimiya, 57(1), 77-84.